2xsv

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{{Seed}}
 
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[[Image:2xsv.jpg|left|200px]]
 
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==Crystal structure of L69A mutant Acinetobacter radioresistens catechol 1,2 dioxygenase==
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The line below this paragraph, containing "STRUCTURE_2xsv", creates the "Structure Box" on the page.
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<StructureSection load='2xsv' size='340' side='right'caption='[[2xsv]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xsv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_radioresistens Acinetobacter radioresistens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XSV FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=PIE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOINOSITOL'>PIE</scene></td></tr>
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{{STRUCTURE_2xsv| PDB=2xsv | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xsv OCA], [https://pdbe.org/2xsv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xsv RCSB], [https://www.ebi.ac.uk/pdbsum/2xsv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xsv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9F103_ACIRA Q9F103_ACIRA]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Intradiol-cleaving catechol 1,2 dioxygenases are Fe(III) dependent enzymes that act on catechol and substituted catechols, including chlorocatechols pollutants, by inserting molecular oxygen in the aromatic ring. Members of this class are the object of intense biochemical investigations aimed at the understanding of their catalytic mechanism, particularly for designing mutants with selected catalytic properties. We report here an in depth investigation of catechol 1,2 dioxygenase IsoB from Acinetobacter radioresistens LMG S13 and its A72G and L69A mutants. By applying a multidisciplinary approach that includes high resolution X-rays crystallography, mass spectrometry and single crystal microspectrophotometry, we characterised the phospholipid bound to the enzyme and provided a structural framework to understand the inversion of substrate specificity showed by the mutants. Our results might be of help for the rational design of enzyme mutants showing a biotechnologically relevant substrate specificity, particularly to be used in bioremediation.
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===CRYSTAL STRUCTURE OF WILD TYPE ACINETOBACTER RADIORESISTENS CATECHOL 1,2 DIOXYGENASE===
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X-ray crystallography, mass spectrometry and single crystal microspectrophotometry: A multidisciplinary characterization of catechol 1,2 dioxygenase.,Micalella C, Martignon S, Bruno S, Pioselli B, Caglio R, Valetti F, Pessione E, Giunta C, Rizzi M Biochim Biophys Acta. 2010 Sep 22. PMID:20869471<ref>PMID:20869471</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xsv" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20869471}}, adds the Publication Abstract to the page
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*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20869471 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20869471}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2XSV is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Acinetobacter_radioresistens Acinetobacter radioresistens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSV OCA].
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==Reference==
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<ref group="xtra">PMID:20869471</ref><references group="xtra"/>
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[[Category: Acinetobacter radioresistens]]
[[Category: Acinetobacter radioresistens]]
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[[Category: Bruno, S.]]
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[[Category: Large Structures]]
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[[Category: Martignon, S.]]
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[[Category: Bruno S]]
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[[Category: Micalella, C.]]
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[[Category: Martignon S]]
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[[Category: Rizzi, M.]]
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[[Category: Micalella C]]
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[[Category: Oxidoreductase]]
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[[Category: Rizzi M]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 13 10:46:15 2010''
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Current revision

Crystal structure of L69A mutant Acinetobacter radioresistens catechol 1,2 dioxygenase

PDB ID 2xsv

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