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3boy

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Crystal structure of the HutP antitermination complex bound to the HUT mRNA

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Retrieved from "http://52.214.119.220/wiki/index.php/3boy"

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-[[Image:3boy.jpg|left|200px]]<br /><applet load="3boy" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="3boy, resolution 1.70&Aring;" />
-'''Crystal structure of the HutP antitermination complex bound to the HUT mRNA'''<br />
-==Overview==
+==Crystal structure of the HutP antitermination complex bound to the HUT mRNA==
-HutP is an L-histidine-activated RNA binding protein that regulates the, expression of the histidine utilization (hut) operon in Bacillus subtilis, by binding to cis-acting regulatory sequences on the hut mRNA. The crystal, structure of HutP complexed with an L-histidine analog showed a novel, fold; there are four antiparallel beta strands in the central region of, each monomer, with two alpha helices each on the front and back. Two HutP, monomers form a dimer, and three dimers are arranged in crystallographic, 3-fold symmetry to form a hexamer. A histidine analog was located in, between the two monomers of HutP, with the imidazole group of L-histidine, hydrogen bonded to Glu81. An activation mechanism is proposed based on the, identification of key residues of HutP. The HutP binding region in hut, mRNA was defined: it consists of three UAG trinucleotide motifs separated, by four spacer nucleotides. Residues of HutP potentially important for RNA, binding were identified.
+<StructureSection load='3boy' size='340' side='right'caption='[[3boy]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[3boy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2gzt 2gzt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BOY FirstGlance]. <br>
-BOY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=HIS:'>HIS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 2GZT. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BOY OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3boy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3boy OCA], [https://pdbe.org/3boy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3boy RCSB], [https://www.ebi.ac.uk/pdbsum/3boy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3boy ProSAT], [https://www.topsan.org/Proteins/RSGI/3boy TOPSAN]</span></td></tr>
-Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15242603 15242603]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/HUTP_BACSU HUTP_BACSU] Antiterminator that binds to cis-acting regulatory sequences on the mRNA in the presence of histidine, thereby suppressing transcription termination and activating the hut operon for histidine utilization.[HAMAP-Rule:MF_00779]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/3boy_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3boy ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
 [[Category: Bacillus subtilis]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Balasundaresan, D.]]
+[[Category: Balasundaresan D]]
-[[Category: Jeyakanthan, J.]]
+[[Category: Jeyakanthan J]]
-[[Category: Kumar, P.K.R.]]
+[[Category: Kumar PKR]]
-[[Category: Kumarevel, T.S.]]
+[[Category: Kumarevel TS]]
-[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
+[[Category: Shinkai A]]
-[[Category: Shinkai, A.]]
+[[Category: Yokoyama S]]
-[[Category: Yokoyama, S.]]
-[[Category: HIS]]
-[[Category: MG]]
-[[Category: activator]]
-[[Category: anti-termination]]
-[[Category: histidine metabolism]]
-[[Category: hutp]]
-[[Category: hutp-rna complex]]
-[[Category: national project on protein structural and functional analyses]]
-[[Category: nppsfa]]
-[[Category: riken structural genomics/proteomics initiative]]
-[[Category: rna-binding]]
-[[Category: rsgi]]
-[[Category: structural genomics]]
-[[Category: transcription regulation]]
-[[Category: transcription/rna complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:11:47 2008''

3boy

From Proteopedia

Current revision

Crystal structure of the HutP antitermination complex bound to the HUT mRNA

Structural highlights

Function

Evolutionary Conservation

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