2bw1

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[[Image:2bw1.gif|left|200px]]<br />
 
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<applet load="2bw1" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bw1, resolution 1.81&Aring;" />
 
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'''IRON-BOUND CRYSTAL STRUCTURE OF DPS-LIKE PEROXIDE RESISTANCE PROTEIN (DPR) FROM STREPTOCOCCUS SUIS.'''<br />
 
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==Overview==
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==Iron-bound crystal structure of Dps-like peroxide resistance protein (Dpr) from Streptococcus suis.==
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The Dps-like peroxide resistance protein (Dpr) is a dodecameric protein, that protects the human and swine pathogen Streptococcus suis from, hydrogen peroxide by removing free Fe2+ from the cytosol. Subsequent, oxidation of iron by Dpr results in the deposition of Fe3+ inside the, protein's central cavity. Structural changes that occur in the ferroxidase, center were studied by X-ray crystallography after soaking Dpr crystals, with Fe2+ in the presence of sodium dithionite. Twelve iron-binding sites, were identified with each site formed by residues Asp74 and Glu78 from one, subunit, and Asp63, His47 and His59 from a 2-fold symmetry-related, subunit. Compared to the iron-free Dpr, Asp74 and Glu78 were found to be, the most flexible amino acid residues and able to adopt a variety of, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?16997323 (full description)]]
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<StructureSection load='2bw1' size='340' side='right'caption='[[2bw1]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2bw1]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_suis Streptococcus suis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BW1 FirstGlance]. <br>
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2BW1 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptococcus_suis Streptococcus suis]] with FE, CA and EPE as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: FEA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BW1 OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bw1 OCA], [https://pdbe.org/2bw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bw1 RCSB], [https://www.ebi.ac.uk/pdbsum/2bw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bw1 ProSAT]</span></td></tr>
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Iron incorporation in Streptococcus suis Dps-like peroxide resistance protein Dpr requires mobility in the ferroxidase center and leads to the formation of a ferrihydrite-like core., Kauko A, Pulliainen AT, Haataja S, Meyer-Klaucke W, Finne J, Papageorgiou AC, J Mol Biol. 2006 Nov 17;364(1):97-109. Epub 2006 Aug 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16997323 16997323]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/DPS_STRSU DPS_STRSU] Protects DNA from oxidative damage by sequestering intracellular Fe(2+) ion and storing it in the form of Fe(3+) oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe(2+) ions, which prevents hydroxyl radical production by the Fenton reaction (By similarity). It binds and incorporates Fe(2+) iron. Is responsible for hydrogen peroxide resistance. Does not bind DNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/2bw1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bw1 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptococcus suis]]
[[Category: Streptococcus suis]]
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[[Category: Finne, J.]]
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[[Category: Finne J]]
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[[Category: Haataja, S.]]
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[[Category: Haataja S]]
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[[Category: Kauko, A.]]
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[[Category: Kauko A]]
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[[Category: Papageorgiou, A.C.]]
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[[Category: Papageorgiou AC]]
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[[Category: Pulliainen, A.]]
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[[Category: Pulliainen A]]
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[[Category: CA]]
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[[Category: EPE]]
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[[Category: FE]]
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[[Category: dpr]]
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[[Category: dps-family]]
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[[Category: ferritin-like]]
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[[Category: ferroxidase]]
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[[Category: iron-binding]]
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[[Category: peroxide resistance]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:04:27 2007''
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Current revision

Iron-bound crystal structure of Dps-like peroxide resistance protein (Dpr) from Streptococcus suis.

PDB ID 2bw1

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OCA

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