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2jub

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(New page: 200px<br /><applet load="2jub" size="350" color="white" frame="true" align="right" spinBox="true" caption="2jub" /> '''Solution structure of IPI*'''<br /> ==Overv...)
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[[Image:2jub.jpg|left|200px]]<br /><applet load="2jub" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2jub" />
 
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'''Solution structure of IPI*'''<br />
 
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==Overview==
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==Solution structure of IPI*==
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Phage T4 protects its DNA from the two-gene-encoded gmrS/gmrD, (glucose-modified hydroxymethylcytosine restriction endonuclease) CT of, pathogenic Escherichia coli, CT596, by injecting several hundred copies of, the 76-amino-acid-residue nuclease inhibitor, IPI*, into the infected, host. Here, the three-dimensional solution structure of mature IPI* is, reported as determined by nuclear magnetic resonance techniques using 1290, experimental nuclear Overhauser effect and dipolar coupling constraints (, approximately 17 constraints per residue). Close examination of this, oblate-shaped protein structure reveals a novel fold consisting of two, small beta-sheets (beta1: B1 and B2; beta2: B3-B5) flanked at the N- and, C-termini by alpha-helices (H1 and H2). Such a fold is very compact in, shape and allows ejection of IPI* through the narrow 30-A portal and tail, tube apertures of the virion without unfolding. Structural and dynamic, measurements identify an exposed hydrophobic knob that is a putative, gmrS/gmrD-binding site. A single gene from the uropathogenic E. coli, UT189, which codes for a gmrS/gmrD-like UT fusion enzyme (with, approximately 90% identity to the heterodimeric CT enzyme), has evolved, IPI* inhibitor immunity. Analysis of the gmrS/gmrD restriction, endonuclease enzyme family and its IPI* family phage antagonists reveals, an evolutionary pathway that has elaborated a surprisingly diverse and, specifically fitted set of coevolving attack and defense structures.
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<StructureSection load='2jub' size='340' side='right'caption='[[2jub]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jub]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JUB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jub OCA], [https://pdbe.org/2jub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jub RCSB], [https://www.ebi.ac.uk/pdbsum/2jub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jub ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IPI1_BPT4 IPI1_BPT4] Internal protein I is one of four proteins in a complex that functions in bacteriophage head maturation.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phage T4 protects its DNA from the two-gene-encoded gmrS/gmrD (glucose-modified hydroxymethylcytosine restriction endonuclease) CT of pathogenic Escherichia coli, CT596, by injecting several hundred copies of the 76-amino-acid-residue nuclease inhibitor, IPI*, into the infected host. Here, the three-dimensional solution structure of mature IPI* is reported as determined by nuclear magnetic resonance techniques using 1290 experimental nuclear Overhauser effect and dipolar coupling constraints ( approximately 17 constraints per residue). Close examination of this oblate-shaped protein structure reveals a novel fold consisting of two small beta-sheets (beta1: B1 and B2; beta2: B3-B5) flanked at the N- and C-termini by alpha-helices (H1 and H2). Such a fold is very compact in shape and allows ejection of IPI* through the narrow 30-A portal and tail tube apertures of the virion without unfolding. Structural and dynamic measurements identify an exposed hydrophobic knob that is a putative gmrS/gmrD-binding site. A single gene from the uropathogenic E. coli UT189, which codes for a gmrS/gmrD-like UT fusion enzyme (with approximately 90% identity to the heterodimeric CT enzyme), has evolved IPI* inhibitor immunity. Analysis of the gmrS/gmrD restriction endonuclease enzyme family and its IPI* family phage antagonists reveals an evolutionary pathway that has elaborated a surprisingly diverse and specifically fitted set of coevolving attack and defense structures.
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==About this Structure==
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Restriction endonuclease inhibitor IPI* of bacteriophage T4: a novel structure for a dedicated target.,Rifat D, Wright NT, Varney KM, Weber DJ, Black LW J Mol Biol. 2008 Jan 18;375(3):720-34. Epub 2007 Nov 1. PMID:18037438<ref>PMID:18037438</ref>
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2JUB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_tw28 Enterobacteria phage tw28]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUB OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Restriction Endonuclease Inhibitor IPI* of Bacteriophage T4: A Novel Structure for a Dedicated Target., Rifat D, Wright NT, Varney KM, Weber DJ, Black LW, J Mol Biol. 2007 Nov 1;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18037438 18037438]
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</div>
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[[Category: Enterobacteria phage tw28]]
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<div class="pdbe-citations 2jub" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Black, L.W.]]
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<references/>
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[[Category: Rifat, D.]]
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__TOC__
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[[Category: Varney, K.M.]]
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</StructureSection>
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[[Category: Weber, D.J.]]
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[[Category: Escherichia virus T4]]
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[[Category: Wright, N.T.]]
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[[Category: Large Structures]]
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[[Category: endonuclease inhibitor]]
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[[Category: Black LW]]
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[[Category: ipi*]]
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[[Category: Rifat D]]
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[[Category: solution]]
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[[Category: Varney KM]]
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[[Category: t4 phage]]
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[[Category: Weber DJ]]
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[[Category: Wright NT]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:12:54 2008''
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Current revision

Solution structure of IPI*

PDB ID 2jub

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