User:Amy Kerzmann/Sandbox 101910
From Proteopedia
< User:Amy Kerzmann(Difference between revisions)
| (14 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | Actin Structure | + | == '''Actin Structure''' == |
| + | |||
<applet load='3mfp' size='300' frame='true' align='left' caption='Monomeric G-Actin Structure' /> | <applet load='3mfp' size='300' frame='true' align='left' caption='Monomeric G-Actin Structure' /> | ||
| - | <applet load='3mfp.pdb' size='300' frame='true' align='right' caption='Proposed F-Actin Structure' /> | + | <applet load='3mfp.pdb' size='300' frame='true' align='right' scene='User:Amy_Kerzmann/Sandbox_101910/Actin_filament/1' target='1' caption='Proposed F-Actin Structure' /> |
| + | |||
| + | |||
| + | '''Analyzing the Importance of Residue 204''' | ||
| + | |||
| + | A204 is a key residue in the structure of actin. Highlight A204 <scene name='User:Amy_Kerzmann/Sandbox_101910/G-actin/1' target='0' >in G-actin</scene> and <scene name='User:Amy_Kerzmann/Sandbox_101910/Actin_filament/3' target='1' >in F-actin </scene> | ||
| + | |||
| + | The filamentous structure of F-actin is made of individual G-actin subunits bound to ADP. Click on the Jmol menu in the lower right-hand corner of the F-actin window. Select "Main Menu" then "All 5 models". Select the "1.3: 3" option. Return to the Jmol menu. Select "Main Menu" then "Zoom" then "200%". Now you can see the G-actin subunit that looks identical to the one in the window to the left. | ||
| + | |||
| + | To examine the interface between two G-actin monomeric subunits, | ||
| + | <scene name='User:Amy_Kerzmann/Sandbox_101910/Actin_filament-just_2/1' target='1' >click here</scene>. Now <scene name='User:Amy_Kerzmann/Sandbox_101910/Actin_filament-just_2/3' target='1' >display molecular surface</scene> of the upper subunit. <scene name='User:Amy_Kerzmann/Sandbox_101910/Actin_filament-just_2/2' target='1' >Label Y166, D286 and D288</scene>, which are involved in contacts near A204. | ||
Current revision
Actin Structure
|
|
Analyzing the Importance of Residue 204
A204 is a key residue in the structure of actin. Highlight A204 and
The filamentous structure of F-actin is made of individual G-actin subunits bound to ADP. Click on the Jmol menu in the lower right-hand corner of the F-actin window. Select "Main Menu" then "All 5 models". Select the "1.3: 3" option. Return to the Jmol menu. Select "Main Menu" then "Zoom" then "200%". Now you can see the G-actin subunit that looks identical to the one in the window to the left.
To examine the interface between two G-actin monomeric subunits, . Now of the upper subunit. , which are involved in contacts near A204.
