2p3m

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(New page: 200px<br /><applet load="2p3m" size="350" color="white" frame="true" align="right" spinBox="true" caption="2p3m" /> '''Solution structure of Mj0056'''<br /> ==Ove...)
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[[Image:2p3m.jpg|left|200px]]<br /><applet load="2p3m" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p3m" />
 
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'''Solution structure of Mj0056'''<br />
 
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==Overview==
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==Solution structure of Mj0056==
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Proteins of the cradle-loop barrel metafold are formed by duplication of a, conserved betaalphabeta-element, suggesting a common evolutionary origin, from an ancestral group of nucleic acid-binding proteins. The basal fold, within this metafold, the RIFT barrel, is also found in a wide range of, enzymes, whose homologous relationship with the nucleic acid-binding group, is unclear. We have characterized a protein family that is intermediate in, sequence and structure between the basal group of cradle-loop barrels and, one family of RIFT-barrel enzymes, the riboflavin kinases. We report the, structure, substrate-binding mode, and catalytic activity for one of these, proteins, Methanocaldococcus jannaschii Mj0056, which is an archaeal, riboflavin kinase. Mj0056 is unusual in utilizing CTP rather than ATP as, the donor nucleotide, and sequence conservation in the relevant residues, suggests that this is a general feature of archaeal riboflavin kinases.
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<StructureSection load='2p3m' size='340' side='right'caption='[[2p3m]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2p3m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P3M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P3M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p3m OCA], [https://pdbe.org/2p3m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p3m RCSB], [https://www.ebi.ac.uk/pdbsum/2p3m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p3m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIFK_METJA RIFK_METJA] Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN). Can also utilize UTP as the phosphate donor, although less efficiently, and it is unclear if ATP and GTP can also serve as substrates (PubMed:18245297) or not (PubMed:18073108).<ref>PMID:18073108</ref> <ref>PMID:18245297</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p3/2p3m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p3m ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Proteins of the cradle-loop barrel metafold are formed by duplication of a conserved betaalphabeta-element, suggesting a common evolutionary origin from an ancestral group of nucleic acid-binding proteins. The basal fold within this metafold, the RIFT barrel, is also found in a wide range of enzymes, whose homologous relationship with the nucleic acid-binding group is unclear. We have characterized a protein family that is intermediate in sequence and structure between the basal group of cradle-loop barrels and one family of RIFT-barrel enzymes, the riboflavin kinases. We report the structure, substrate-binding mode, and catalytic activity for one of these proteins, Methanocaldococcus jannaschii Mj0056, which is an archaeal riboflavin kinase. Mj0056 is unusual in utilizing CTP rather than ATP as the donor nucleotide, and sequence conservation in the relevant residues suggests that this is a general feature of archaeal riboflavin kinases.
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==About this Structure==
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A CTP-dependent archaeal riboflavin kinase forms a bridge in the evolution of cradle-loop barrels.,Ammelburg M, Hartmann MD, Djuranovic S, Alva V, Koretke KK, Martin J, Sauer G, Truffault V, Zeth K, Lupas AN, Coles M Structure. 2007 Dec;15(12):1577-90. PMID:18073108<ref>PMID:18073108</ref>
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2P3M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Active as [http://en.wikipedia.org/wiki/Riboflavin_kinase Riboflavin kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.26 2.7.1.26] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P3M OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A CTP-Dependent Archaeal Riboflavin Kinase Forms a Bridge in the Evolution of Cradle-Loop Barrels., Ammelburg M, Hartmann MD, Djuranovic S, Alva V, Koretke KK, Martin J, Sauer G, Truffault V, Zeth K, Lupas AN, Coles M, Structure. 2007 Dec;15(12):1577-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18073108 18073108]
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</div>
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<div class="pdbe-citations 2p3m" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Riboflavin kinase]]
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[[Category: Coles M]]
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[[Category: Single protein]]
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[[Category: Djuranovic S]]
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[[Category: Coles, M.]]
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[[Category: Lupas AN]]
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[[Category: Djuranovic, S.]]
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[[Category: Martin J]]
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[[Category: Lupas, A.N.]]
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[[Category: Truffault V]]
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[[Category: Martin, J.]]
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[[Category: Truffault, V.]]
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[[Category: phosphotransferase]]
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[[Category: riboflavin kinase]]
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[[Category: rift barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:23:03 2008''
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Current revision

Solution structure of Mj0056

PDB ID 2p3m

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