2p7v

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[[Image:2p7v.jpg|left|200px]]<br /><applet load="2p7v" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p7v, resolution 2.600&Aring;" />
 
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'''Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4'''<br />
 
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==Overview==
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==Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4==
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The Escherichia coli Rsd protein binds tightly and specifically to the RNA, polymerase (RNAP) sigma(70) factor. Rsd plays a role in alternative sigma, factor-dependent transcription by biasing the competition between, sigma(70) and alternative sigma factors for the available core RNAP. Here, we determined the 2.6 A-resolution X-ray crystal structure of Rsd bound to, sigma(70) domain 4 (sigma(70)(4)), the primary determinant for Rsd binding, within sigma(70). The structure reveals that Rsd binding interferes with, the two primary functions of sigma(70)(4), core RNAP binding and promoter, -35 element binding. Interestingly, the most highly conserved Rsd residues, form a network of interactions through the middle of the Rsd structure, that connect the sigma(70)(4)-binding surface with three cavities exposed, on distant surfaces of Rsd, suggesting functional coupling between, sigma(70)(4) binding and other binding surfaces of Rsd, either for other, proteins or for as yet unknown small molecule effectors. These results, provide a structural basis for understanding the role of Rsd, as well as, its ortholog, AlgQ, a positive regulator of Pseudomonas aeruginosa, virulence, in transcription regulation.
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<StructureSection load='2p7v' size='340' side='right'caption='[[2p7v]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2p7v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P7V FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p7v OCA], [https://pdbe.org/2p7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p7v RCSB], [https://www.ebi.ac.uk/pdbsum/2p7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p7v ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RSD_ECOLI RSD_ECOLI] Binds RpoD and negatively regulates RpoD-mediated transcription activation by preventing the interaction between the primary sigma factor RpoD with the catalytic core of the RNA polymerase and with promoter DNA. May be involved in replacement of the RNA polymerase sigma subunit from RpoD to RpoS during the transition from exponential growth to the stationary phase.<ref>PMID:9560209</ref> <ref>PMID:10368152</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p7/2p7v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p7v ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2P7V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P7V OCA].
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal Structure of the Escherichia coli Regulator of sigma(70), Rsd, in Complex with sigma(70) Domain 4., Patikoglou GA, Westblade LF, Campbell EA, Lamour V, Lane WJ, Darst SA, J Mol Biol. 2007 Jul 3;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17681541 17681541]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Campbell, E.A.]]
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[[Category: Campbell EA]]
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[[Category: Darst, S.A.]]
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[[Category: Darst SA]]
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[[Category: Lamour, V.]]
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[[Category: Lamour V]]
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[[Category: Lane, W.J.]]
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[[Category: Lane WJ]]
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[[Category: Patikoglou, G.A.]]
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[[Category: Patikoglou GA]]
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[[Category: Westblade, L.F.]]
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[[Category: Westblade LF]]
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[[Category: MG]]
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[[Category: e. coli rsd-sigma 70 complex]]
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[[Category: escherichia coli rsd-sigma 70 complex]]
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[[Category: helix-turn-helix]]
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[[Category: regulation]]
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[[Category: regulator of sigma 70]]
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[[Category: rsd]]
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[[Category: rsd-sigma 70 complex]]
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[[Category: sigma 70]]
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[[Category: sigma 70 domain 4]]
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[[Category: sigma factor]]
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[[Category: transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:23:04 2008''
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Current revision

Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4

PDB ID 2p7v

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