We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2pft

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:09, 21 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2pft.gif|left|200px]]<br /><applet load="2pft" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2pft, resolution 2.250&Aring;" />
 
-
'''The Crystal Structure of Mouse Exo70 Reveals Unique Features of the Mammalian Exocyst'''<br />
 
-
==Overview==
+
==The Crystal Structure of Mouse Exo70 Reveals Unique Features of the Mammalian Exocyst==
-
The exocyst is a eukaryotic tethering complex necessary for the fusion of, exocytic vesicles with the plasma membrane. Its function in vivo is, tightly regulated by interactions with multiple small GTPases. Exo70, one, of the eight subunits of the exocyst, is important for the localization of, the exocyst to the plasma membrane. It interacts with TC10 and Rho3, GTPases in mammals and yeast, respectively, and has been shown recently to, bind to the actin-polymerization complex Arp2/3. Here, we present the, crystal structure of Mus musculus Exo70 at 2.25 A resolution. Exo70 is, composed of alpha-helices in a series of right-handed helix-turn-helix, motifs organized into a long rod of length 170 A and width 35 A. Although, the alpha-helical organization of this molecule is similar to that in, Saccharomyces cerevisiae Exo70, major structural differences are observed, on the surface of the molecule, at the domain boundaries, and in various, loop structures. In particular, the C-terminal domain of M. musculus Exo70, adopts a new orientation relative to the N-terminal half not seen in S., cerevisiae Exo70 structures. Given the low level of sequence conservation, within Exo70, this structure provides new insights into our understanding, of many species-specific functions of the exocyst.
+
<StructureSection load='2pft' size='340' side='right'caption='[[2pft]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[2pft]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PFT FirstGlance]. <br>
-
2PFT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFT OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pft OCA], [https://pdbe.org/2pft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pft RCSB], [https://www.ebi.ac.uk/pdbsum/2pft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pft ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
The crystal structure of mouse Exo70 reveals unique features of the mammalian exocyst., Moore BA, Robinson HH, Xu Z, J Mol Biol. 2007 Aug 10;371(2):410-21. Epub 2007 May 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17583731 17583731]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/EXOC7_MOUSE EXOC7_MOUSE] Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion. It is required for neuron survival and plays an essential role in cortical development (By similarity).[UniProtKB:E7FC72]<ref>PMID:12687004</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pf/2pft_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pft ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Single protein]]
+
[[Category: Moore BA]]
-
[[Category: Moore, B.A.]]
+
[[Category: Xu Z]]
-
[[Category: Xu, Z.]]
+
-
[[Category: endocytosis/exocytosis complex]]
+
-
[[Category: helix-turn-helix]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:23:12 2008''
+

Current revision

The Crystal Structure of Mouse Exo70 Reveals Unique Features of the Mammalian Exocyst

PDB ID 2pft

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2pft"
Personal tools