2xql

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (18:16, 18 October 2023) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2xql.jpg|left|200px]]
 
-
<!--
+
==Fitting of the H2A-H2B histones in the electron microscopy map of the complex Nucleoplasmin:H2A-H2B histones (1:5).==
-
The line below this paragraph, containing "STRUCTURE_2xql", creates the "Structure Box" on the page.
+
<SX load='2xql' size='340' side='right' viewer='molstar' caption='[[2xql]], [[Resolution|resolution]] 19.50&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2xql]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XQL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XQL FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 19.5&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xql OCA], [https://pdbe.org/2xql PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xql RCSB], [https://www.ebi.ac.uk/pdbsum/2xql PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xql ProSAT]</span></td></tr>
-
{{STRUCTURE_2xql| PDB=2xql | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/H2A4_CHICK H2A4_CHICK] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Nucleoplasmin (NP) is a pentameric chaperone that regulates the condensation state of chromatin extracting specific basic proteins from sperm chromatin and depositing H2A-H2B histone dimers. It has been proposed that histones could bind to either the lateral or distal face of the pentameric structure. Here, we combine different biochemical and biophysical techniques to show that natural, hyperphosphorylated NP can bind five H2A-H2B dimers and that the amount of bound ligand depends on the overall charge (phosphorylation level) of the chaperone. Three-dimensional reconstruction of NP/H2A-H2B complex carried out by electron microscopy reveals that histones interact with the chaperone distal face. Limited proteolysis and mass spectrometry indicate that the interaction results in protection of the histone fold and most of the H2A and H2B C-terminal tails. This structural information can help to understand the function of NP as a histone chaperone.
-
===FITTING OF THE H2A-H2B HISTONES IN THE ELECTRON MICROSCOPY MAP OF THE COMPLEX NUCLEOPLASMIN:H2A-H2B HISTONES (1:5).===
+
Nucleoplasmin binds histone H2A-H2B dimers through its distal face.,Ramos I, Martin-Benito J, Finn R, Bretana L, Aloria K, Arizmendi JM, Ausio J, Muga A, Valpuesta JM, Prado A J Biol Chem. 2010 Oct 29;285(44):33771-8. Epub 2010 Aug 9. PMID:20696766<ref>PMID:20696766</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2xql" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_20696766}}, adds the Publication Abstract to the page
+
*[[Histone 3D structures|Histone 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 20696766 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_20696766}}
+
__TOC__
-
 
+
</SX>
-
==About this Structure==
+
-
2XQL is a 10 chains structure with sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XQL OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:20696766</ref><references group="xtra"/>
+
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
-
[[Category: Aloria, K.]]
+
[[Category: Large Structures]]
-
[[Category: Arizmendi, J M.]]
+
[[Category: Aloria K]]
-
[[Category: Ausio, J.]]
+
[[Category: Arizmendi JM]]
-
[[Category: Bretana, L.]]
+
[[Category: Ausio J]]
-
[[Category: Finn, R.]]
+
[[Category: Bretana L]]
-
[[Category: Martin-Benito, J.]]
+
[[Category: Finn R]]
-
[[Category: Muga, A.]]
+
[[Category: Martin-Benito J]]
-
[[Category: Prado, A.]]
+
[[Category: Muga A]]
-
[[Category: Ramos, I.]]
+
[[Category: Prado A]]
-
[[Category: Valpuesta, J M.]]
+
[[Category: Ramos I]]
-
[[Category: Chaperone]]
+
[[Category: Valpuesta JM]]
-
[[Category: Chromatin]]
+
-
[[Category: Histone-chaperone]]
+
-
[[Category: Nuclear protein]]
+
-
[[Category: Nuclear-chaperone]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 3 11:06:17 2010''
+

Current revision

Fitting of the H2A-H2B histones in the electron microscopy map of the complex Nucleoplasmin:H2A-H2B histones (1:5).

2xql, resolution 19.50Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xql"
Personal tools