3ahf

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{{Seed}}
 
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[[Image:3ahf.png|left|200px]]
 
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==Phosphoketolase from Bifidobacterium Breve complexed with inorganic phosphate==
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The line below this paragraph, containing "STRUCTURE_3ahf", creates the "Structure Box" on the page.
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<StructureSection load='3ahf' size='340' side='right'caption='[[3ahf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ahf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium_breve Bifidobacterium breve]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AHF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
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{{STRUCTURE_3ahf| PDB=3ahf | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ahf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ahf OCA], [https://pdbe.org/3ahf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ahf RCSB], [https://www.ebi.ac.uk/pdbsum/3ahf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ahf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D6PAH1_BIFBR D6PAH1_BIFBR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/3ahf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ahf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Thiamine diphosphate (ThDP)-dependent enzymes are ubiquitously present in all organisms and catalyze essential reactions in various metabolic pathways. ThDP-dependent phosphoketolase plays key roles in the central metabolism of heterofermentative bacteria and in the pentose catabolism of various microbes. In particular, bifidobacteria, representatives of beneficial commensal bacteria, have an effective glycolytic pathway called bifid shunt in which 2.5 mol of ATP are produced per glucose. Phosphoketolase catalyzes two steps in the bifid shunt because of its dual-substrate specificity; they are phosphorolytic cleavage of fructose 6-phosphate or xylulose 5-phosphate to produce aldose phosphate, acetyl phosphate, and H(2)O. The phosphoketolase reaction is different from other well studied ThDP-dependent enzymes because it involves a dehydration step. Although phosphoketolase was discovered more than 50 years ago, its three-dimensional structure remains unclear. In this study we report the crystal structures of xylulose 5-phosphate/fructose 6-phosphate phosphoketolase from Bifidobacterium breve. The structures of the two intermediates before and after dehydration (alpha,beta-dihydroxyethyl ThDP and 2-acetyl-ThDP) and complex with inorganic phosphate give an insight into the mechanism of each step of the enzymatic reaction.
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===Phosphoketolase from Bifidobacterium Breve complexed with inorganic phosphate===
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Crystal structures of phosphoketolase: thiamine diphosphate-dependent dehydration mechanism.,Suzuki R, Katayama T, Kim BJ, Wakagi T, Shoun H, Ashida H, Yamamoto K, Fushinobu S J Biol Chem. 2010 Oct 29;285(44):34279-87. Epub 2010 Aug 24. PMID:20739284<ref>PMID:20739284</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20739284}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3ahf" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20739284 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20739284}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3AHF is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bifidobacterium_breve Bifidobacterium breve]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AHF OCA].
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==Reference==
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<ref group="xtra">PMID:20739284</ref><ref group="xtra">PMID:20693675</ref><references group="xtra"/>
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[[Category: Bifidobacterium breve]]
[[Category: Bifidobacterium breve]]
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[[Category: Fructose-6-phosphate phosphoketolase]]
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[[Category: Large Structures]]
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[[Category: Ashida, H.]]
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[[Category: Ashida H]]
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[[Category: Fushinobu, S.]]
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[[Category: Fushinobu S]]
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[[Category: Katayama, T.]]
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[[Category: Katayama T]]
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[[Category: Kim, B J.]]
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[[Category: Kim B-J]]
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[[Category: Shoun, H.]]
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[[Category: Shoun H]]
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[[Category: Suzuki, R.]]
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[[Category: Suzuki R]]
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[[Category: Wakagi, T.]]
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[[Category: Wakagi T]]
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[[Category: Yamamoto, K.]]
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[[Category: Yamamoto K]]
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[[Category: Alpha-beta fold]]
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[[Category: Homodimer]]
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[[Category: Inorganic phosphate]]
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[[Category: Lyase]]
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[[Category: Thiamine diphosphate-dependent enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 3 11:19:31 2010''
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Current revision

Phosphoketolase from Bifidobacterium Breve complexed with inorganic phosphate

PDB ID 3ahf

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