2ze0

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(New page: 200px<br /><applet load="2ze0" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ze0, resolution 2.00&Aring;" /> '''Alpha-glucosidase GS...)
Current revision (13:33, 1 November 2023) (edit) (undo)
 
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[[Image:2ze0.jpg|left|200px]]<br /><applet load="2ze0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ze0, resolution 2.00&Aring;" />
 
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'''Alpha-glucosidase GSJ'''<br />
 
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==Overview==
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==Alpha-glucosidase GSJ==
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An alpha-glucosidase (EC 3.2.1.20) was purified from Geobacillus sp., strain HTA-462 cells and crystallized using the hanging-drop, vapour-diffusion technique. The Geobacillus strain is a thermophilic and, high-pressure-resistant bacterium found at the bottom of the Challenger, Deep in the Mariana Trench. The crystal was characterized by X-ray, diffraction and belongs to space group C2, with unit-cell parameters a =, 104.0, b = 91.5, c = 72.9 A, beta = 109.4 degrees. Diffraction data to 2.5, A resolution were collected and processed.
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<StructureSection load='2ze0' size='340' side='right'caption='[[2ze0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ze0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_sp._HTA-462 Geobacillus sp. HTA-462]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZE0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZE0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ze0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ze0 OCA], [https://pdbe.org/2ze0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ze0 RCSB], [https://www.ebi.ac.uk/pdbsum/2ze0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ze0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q33E90_9BACI Q33E90_9BACI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ze/2ze0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ze0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the GH13 alpha-glucosidase (GSJ) from deep-sea bacterium Geobacillus sp. strain HTA-462 was determined to a 2.0 A resolution. Comparisons of the GSJ structure with that of other GH13 enzymes with different catalytic activities revealed that the catalytic cleft of GSJ was widely opened when compared with the homologues. The wide opening of the catalytic cleft originated from conformational changes of active site residues and disorder of the regions close to the catalytic center. This structural feature of GSJ would explain the ability of this enzyme to accept a wide variety of nonsugar molecules as acceptors in the transglycosylation reaction.
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==About this Structure==
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Crystal structure of GH13 alpha-glucosidase GSJ from one of the deepest sea bacteria.,Shirai T, Hung VS, Morinaka K, Kobayashi T, Ito S Proteins. 2008 Oct;73(1):126-33. PMID:18398906<ref>PMID:18398906</ref>
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2ZE0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_sp. Geobacillus sp.] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Alpha-glucosidase Alpha-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.20 3.2.1.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZE0 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystallization and preliminary X-ray study of alpha-glucosidase from Geobacillus sp strain HTA-462, one of the deepest sea bacteria., Shirai T, Hung VS, Akita M, Hatada Y, Ito S, Horikoshi K, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1278-9. Epub 2003, Jun 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12832785 12832785]
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</div>
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[[Category: Alpha-glucosidase]]
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<div class="pdbe-citations 2ze0" style="background-color:#fffaf0;"></div>
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[[Category: Geobacillus sp.]]
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[[Category: Single protein]]
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[[Category: Hung, V.S.]]
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[[Category: Ito, S.]]
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[[Category: Kobayashi, T.]]
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[[Category: Morinaka, K.]]
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[[Category: Shirai, T.]]
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[[Category: CA]]
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[[Category: extremophile]]
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[[Category: glucoside hydrolase]]
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[[Category: tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:36:45 2008''
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==See Also==
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*[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Geobacillus sp. HTA-462]]
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[[Category: Large Structures]]
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[[Category: Hung VS]]
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[[Category: Ito S]]
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[[Category: Kobayashi T]]
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[[Category: Morinaka K]]
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[[Category: Shirai T]]

Current revision

Alpha-glucosidase GSJ

PDB ID 2ze0

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