2pex

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(New page: 200px<br /><applet load="2pex" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pex, resolution 1.900&Aring;" /> '''Structure of reduce...)
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[[Image:2pex.jpg|left|200px]]<br /><applet load="2pex" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2pex, resolution 1.900&Aring;" />
 
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'''Structure of reduced C22S OhrR from Xanthamonas Campestris'''<br />
 
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==Overview==
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==Structure of reduced C22S OhrR from Xanthamonas Campestris==
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The Xanthomonas campestris transcription regulator OhrR contains a, reactive cysteine residue (C22) that upon oxidation by organic, hydroperoxides (OHPs) forms an intersubunit disulphide bond with residue, C127'. Such modification induces the expression of a peroxidase that, reduces OHPs to their less toxic alcohols. Here, we describe the, structures of reduced and OHP-oxidized OhrR, visualizing the structural, mechanism of OHP induction. Reduced OhrR takes a canonical MarR family, fold with C22 and C127' separated by 15.5 A. OHP oxidation results in the, disruption of the Y36'-C22-Y47' interaction network and dissection of, helix alpha5, which then allows the 135 degrees rotation and 8.2 A, translation of C127', formation of the C22-C127' disulphide bond, and, alpha6-alpha6' helix-swapped reconfiguration of the dimer interface. These, changes result in the 28 degrees rigid body rotations of each winged, helix-turn-helix motif and DNA dissociation. Similar effector-induced, rigid body rotations are expected for most MarR family members.
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<StructureSection load='2pex' size='340' side='right'caption='[[2pex]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2pex]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PEX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pex OCA], [https://pdbe.org/2pex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pex RCSB], [https://www.ebi.ac.uk/pdbsum/2pex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pex ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q93R11_XANCH Q93R11_XANCH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pe/2pex_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pex ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2PEX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris] with <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEX OCA].
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*[[Organic hydroperoxide resistance protein|Organic hydroperoxide resistance protein]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural Mechanism of Organic Hydroperoxide Induction of the Transcription Regulator OhrR., Newberry KJ, Fuangthong M, Panmanee W, Mongkolsuk S, Brennan RG, Mol Cell. 2007 Nov 30;28(4):652-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18042459 18042459]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Xanthomonas campestris]]
[[Category: Xanthomonas campestris]]
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[[Category: Brennan, R.G.]]
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[[Category: Brennan RG]]
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[[Category: Newberry, K.J.]]
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[[Category: Newberry KJ]]
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[[Category: FMT]]
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[[Category: transcription regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:43:39 2008''
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Current revision

Structure of reduced C22S OhrR from Xanthamonas Campestris

PDB ID 2pex

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