2xgq

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{{Seed}}
 
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[[Image:2xgq.png|left|200px]]
 
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==Structure of yeast DNA polymerase eta in complex with C8-N-acetyl-2- aminoanthracene containing DNA==
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The line below this paragraph, containing "STRUCTURE_2xgq", creates the "Structure Box" on the page.
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<StructureSection load='2xgq' size='340' side='right'caption='[[2xgq]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xgq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XGQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8AG:8-[ACETYL(ANTHRACEN-2-YL)AMINO]-2-DEOXYGUANOSINE+5-(DIHYDROGEN+PHOSPHATE)'>8AG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_2xgq| PDB=2xgq | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xgq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xgq OCA], [https://pdbe.org/2xgq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xgq RCSB], [https://www.ebi.ac.uk/pdbsum/2xgq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xgq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/POLH_YEAST POLH_YEAST] DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. Efficiently incorporates nucleotides opposite to other UV or oxidative DNA damages like O(6)-methylguanine, 7,8-dihydro-8-oxoguanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine of 2'-deoxyguanosine (FaPydG), or p-benzoquinone DNA adducts.<ref>PMID:9409821</ref> <ref>PMID:10347143</ref> <ref>PMID:10601233</ref> <ref>PMID:9974380</ref> <ref>PMID:10924462</ref> <ref>PMID:10713149</ref> <ref>PMID:11027270</ref> <ref>PMID:10932195</ref> <ref>PMID:10725365</ref> <ref>PMID:11062246</ref> <ref>PMID:11545742</ref> <ref>PMID:11113193</ref> <ref>PMID:11238937</ref> <ref>PMID:11054429</ref> <ref>PMID:12110599</ref> <ref>PMID:11861920</ref> <ref>PMID:12899630</ref> <ref>PMID:12665597</ref> <ref>PMID:12888515</ref> <ref>PMID:12692307</ref> <ref>PMID:14527996</ref> <ref>PMID:15157108</ref> <ref>PMID:15544332</ref> <ref>PMID:15284331</ref> <ref>PMID:15333698</ref> <ref>PMID:15024063</ref> <ref>PMID:15779911</ref> <ref>PMID:16181813</ref> <ref>PMID:15520252</ref> <ref>PMID:16366567</ref> <ref>PMID:15743815</ref> <ref>PMID:16866379</ref> <ref>PMID:16387871</ref> <ref>PMID:16415180</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Heterocyclic aromatic amines produce bulky C8 guanine lesions in vivo, which interfere and disrupt DNA and RNA synthesis. These lesions are consequently strong replication blocks. In addition bulky adducts give rise to point and frameshift mutations. The translesion synthesis (TLS) DNA polymerase eta is able to bypass slowly C8 bulky adduct lesions such as the widely studied 2-aminofluorene-dG and its acetylated analogue mainly in an error-free manner. Replicative polymerases are in contrast fully blocked by the acetylated lesion. Here, we show that TLS efficiency of Pol eta depends critically on the size of the bulky adduct forming the lesion. Based on the crystal structure, we show why the bypass reaction is so difficult and we provide a model for the bypass reaction. In our model, TLS is accomplished without rotation of the lesion into the anti conformation as previously thought.
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===STRUCTURE OF YEAST DNA POLYMERASE ETA IN COMPLEX WITH C8-N-ACETYL-2-AMINOANTHRACENE CONTAINING DNA===
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Mechanism of replication blocking and bypass of Y-family polymerase {eta} by bulky acetylaminofluorene DNA adducts.,Schorr S, Schneider S, Lammens K, Hopfner KP, Carell T Proc Natl Acad Sci U S A. 2010 Nov 12. PMID:21076032<ref>PMID:21076032</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xgq" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21076032}}, adds the Publication Abstract to the page
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21076032 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21076032}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2XGQ is a 6 chains structure with sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGQ OCA].
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==Reference==
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<ref group="xtra">PMID:21076032</ref><references group="xtra"/>
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Carell, T.]]
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[[Category: Carell T]]
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[[Category: Schneider, S.]]
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[[Category: Schneider S]]
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[[Category: Schorr, S.]]
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[[Category: Schorr S]]
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[[Category: Dna damage]]
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[[Category: Dna-binding]]
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[[Category: Transferase-dna complex]]
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[[Category: Translesion dna synthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 24 13:46:55 2010''
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Current revision

Structure of yeast DNA polymerase eta in complex with C8-N-acetyl-2- aminoanthracene containing DNA

PDB ID 2xgq

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