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2rak

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(New page: 200px<br /><applet load="2rak" size="350" color="white" frame="true" align="right" spinBox="true" caption="2rak, resolution 3.00&Aring;" /> '''PI(3)P bound PX-BAR ...)
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[[Image:2rak.jpg|left|200px]]<br /><applet load="2rak" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2rak, resolution 3.00&Aring;" />
 
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'''PI(3)P bound PX-BAR membrane remodeling unit of Sorting Nexin 9'''<br />
 
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==Overview==
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==PI(3)P bound PX-BAR membrane remodeling unit of Sorting Nexin 9==
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Sorting nexins (SNXs) form a family of proteins known to interact with, components in the endosomal system and to regulate various steps of, vesicle transport. Sorting nexin 9 (SNX9) is involved in the late stages, of clathrin-mediated endocytosis in non-neuronal cells, where together, with the GTPase dynamin, it participates in the formation and scission of, the vesicle neck. We report here crystal structures of the functional, membrane-remodeling unit of SNX9 and show that it efficiently tubulates, lipid membranes in vivo and in vitro. Elucidation of the protein, superdomain structure, together with mutational analysis and biochemical, and cell biological experiments, demonstrated how the SNX9 PX and BAR, domains work in concert in targeting and tubulation of, phosphoinositide-containing membranes. The study provides insights into, the SNX9-induced membrane modulation mechanism.
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<StructureSection load='2rak' size='340' side='right'caption='[[2rak]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2rak]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RAK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PIB:2-(BUTANOYLOXY)-1-{[(HYDROXY{[2,3,4,6-TETRAHYDROXY-5-(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL)OXY]METHYL}ETHYL+BUTANOATE'>PIB</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rak OCA], [https://pdbe.org/2rak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rak RCSB], [https://www.ebi.ac.uk/pdbsum/2rak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rak ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SNX9_HUMAN SNX9_HUMAN] May be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate.<ref>PMID:11799118</ref> <ref>PMID:12952949</ref> <ref>PMID:15703209</ref> <ref>PMID:17609109</ref> <ref>PMID:18388313</ref> <ref>PMID:20427313</ref> <ref>PMID:21048941</ref> <ref>PMID:17948057</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/2rak_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rak ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2RAK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PIB:'>PIB</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RAK OCA].
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*[[Sorting nexin 3D structures|Sorting nexin 3D structures]]
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== References ==
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==Reference==
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<references/>
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The PX-BAR membrane-remodeling unit of sorting nexin 9., Pylypenko O, Lundmark R, Rasmuson E, Carlsson SR, Rak A, EMBO J. 2007 Nov 14;26(22):4788-800. Epub 2007 Oct 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17948057 17948057]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Carlsson, S.R.]]
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[[Category: Carlsson SR]]
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[[Category: Lundmark, R.]]
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[[Category: Lundmark R]]
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[[Category: Pylypenko, O.]]
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[[Category: Pylypenko O]]
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[[Category: Rak, A.]]
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[[Category: Rak A]]
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[[Category: Rasmuson, E.]]
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[[Category: Rasmuson E]]
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[[Category: PIB]]
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[[Category: bar domain]]
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[[Category: membrane transport]]
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[[Category: px domain]]
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[[Category: sorting nexin]]
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[[Category: structural protein]]
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[[Category: tubulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:45:28 2008''
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Current revision

PI(3)P bound PX-BAR membrane remodeling unit of Sorting Nexin 9

PDB ID 2rak

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