3iyw

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{{Seed}}
 
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[[Image:3iyw.jpg|left|200px]]
 
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==West Nile virus in complex with Fab fragments of MAb CR4354 (fitted coordinates of envelope proteins and Fab fragments of one icosahedral ASU)==
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The line below this paragraph, containing "STRUCTURE_3iyw", creates the "Structure Box" on the page.
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<SX load='3iyw' size='340' side='right' viewer='molstar' caption='[[3iyw]], [[Resolution|resolution]] 13.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3iyw]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/West_Nile_virus West Nile virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IYW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IYW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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{{STRUCTURE_3iyw| PDB=3iyw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iyw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iyw OCA], [https://pdbe.org/3iyw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iyw RCSB], [https://www.ebi.ac.uk/pdbsum/3iyw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iyw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q91R02_WNV Q91R02_WNV]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many flaviviruses are significant human pathogens, with the humoral immune response playing an essential role in restricting infection and disease. CR4354, a human monoclonal antibody isolated from a patient, neutralizes West Nile virus (WNV) infection at a postattachment stage in the viral life-cycle. Here, we determined the structure of WNV complexed with Fab fragments of CR4354 using cryoelectron microscopy. The outer glycoprotein shell of a mature WNV particle is formed by 30 rafts of three homodimers of the viral surface protein E. CR4354 binds to a discontinuous epitope formed by protein segments from two neighboring E molecules, but does not cause any detectable structural disturbance on the viral surface. The epitope occurs at two independent positions within an icosahedral asymmetric unit, resulting in 120 binding sites on the viral surface. The cross-linking of the six E monomers within one raft by four CR4354 Fab fragments suggests that the antibody neutralizes WNV by blocking the pH-induced rearrangement of the E protein required for virus fusion with the endosomal membrane.
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===West Nile virus in complex with Fab fragments of MAb CR4354 (fitted coordinates of envelope proteins and Fab fragments of one icosahedral ASU)===
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Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354.,Kaufmann B, Vogt MR, Goudsmit J, Holdaway HA, Aksyuk AA, Chipman PR, Kuhn RJ, Diamond MS, Rossmann MG Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18950-5. Epub 2010 Oct 18. PMID:20956322<ref>PMID:20956322</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3iyw" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20956322}}, adds the Publication Abstract to the page
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20956322 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20956322}}
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__TOC__
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</SX>
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==About this Structure==
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3IYW is a 7 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/West_nile_virus West nile virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IYW OCA].
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==Reference==
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<ref group="xtra">PMID:20956322</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: West nile virus]]
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[[Category: Large Structures]]
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[[Category: Kaufmann, B.]]
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[[Category: West Nile virus]]
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[[Category: Rossmann, M G.]]
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[[Category: Kaufmann B]]
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[[Category: Envelope protein]]
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[[Category: Rossmann MG]]
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[[Category: Flavivirus]]
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[[Category: Icosahedral virus]]
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[[Category: Neutralizing fab fragment]]
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[[Category: Virus]]
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[[Category: Virus-antibody complex]]
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[[Category: West nile virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 24 14:12:47 2010''
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Current revision

West Nile virus in complex with Fab fragments of MAb CR4354 (fitted coordinates of envelope proteins and Fab fragments of one icosahedral ASU)

3iyw, resolution 13.70Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

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