2vb6

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(New page: 200px<br /><applet load="2vb6" size="350" color="white" frame="true" align="right" spinBox="true" caption="2vb6, resolution 2.30&Aring;" /> '''MYOSIN VI (MD-INSERT...)
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[[Image:2vb6.jpg|left|200px]]<br /><applet load="2vb6" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2vb6, resolution 2.30&Aring;" />
 
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'''MYOSIN VI (MD-INSERT2-CAM, DELTA INSERT1) POST-RIGOR STATE (CRYSTAL FORM 2)'''<br />
 
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==Overview==
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==Myosin VI (MD-insert2-CaM, Delta Insert1) Post-rigor state (crystal form 2)==
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Myosin VI has an unexpectedly large swing of its lever arm (powerstroke), that optimizes its unique reverse direction movement. The basis for this, is an unprecedented rearrangement of the subdomain to which the lever arm, is attached, referred to as the converter. It is unclear at what point(s), in the myosin VI ATPase cycle rearrangements in the converter occur, and, how this would effect lever arm position. We solved the structure of, myosin VI with an ATP analogue (ADP.BeF(3)) bound in its, nucleotide-binding pocket. The structure reveals that no rearrangement in, the converter occur upon ATP binding. Based on previously solved myosin, structures, our structure suggests that no reversal of the powerstroke, occurs during detachment of myosin VI from actin. The structure also, reveals novel features of the myosin VI motor that may be important in, maintaining the converter conformation during detachment from actin, and, other features that may promote rapid rearrangements in the structure, following actin detachment that enable hydrolysis of ATP.
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<StructureSection load='2vb6' size='340' side='right'caption='[[2vb6]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2vb6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VB6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vb6 OCA], [https://pdbe.org/2vb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vb6 RCSB], [https://www.ebi.ac.uk/pdbsum/2vb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vb6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYO6_PIG MYO6_PIG] Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Myosin 6 is a reverse-direction motor protein that moves towards the minus-end of actin filaments. Has slow rate of actin-activated ADP release due to weak ATP binding. Functions in a variety of intracellular processes such as vesicular membrane trafficking and cell migration. Required for the structural integrity of the Golgi apparatus via the p53-dependent pro-survival pathway. Appears to be involved in a very early step of clathrin-mediated endocytosis in polarized epithelial cells. May act as a regulator of F-actin dynamics. May play a role in transporting DAB2 from the plasma membrane to specific cellular targets. Required for structural integrity of inner ear hair cells (By similarity).<ref>PMID:16917816</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vb/2vb6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vb6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Myosin VI has an unexpectedly large swing of its lever arm (powerstroke) that optimizes its unique reverse direction movement. The basis for this is an unprecedented rearrangement of the subdomain to which the lever arm is attached, referred to as the converter. It is unclear at what point(s) in the myosin VI ATPase cycle rearrangements in the converter occur, and how this would effect lever arm position. We solved the structure of myosin VI with an ATP analogue (ADP.BeF3) bound in its nucleotide-binding pocket. The structure reveals that no rearrangement in the converter occur upon ATP binding. Based on previously solved myosin structures, our structure suggests that no reversal of the powerstroke occurs during detachment of myosin VI from actin. The structure also reveals novel features of the myosin VI motor that may be important in maintaining the converter conformation during detachment from actin, and other features that may promote rapid rearrangements in the structure following actin detachment that enable hydrolysis of ATP.
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==About this Structure==
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The post-rigor structure of myosin VI and implications for the recovery stroke.,Menetrey J, Llinas P, Cicolari J, Squires G, Liu X, Li A, Sweeney HL, Houdusse A EMBO J. 2008 Jan 9;27(1):244-52. Epub 2007 Nov 29. PMID:18046460<ref>PMID:18046460</ref>
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2VB6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=BEF:'>BEF</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Adp Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Bef Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Mg Binding Site For Chain A'>AC3</scene>, <scene name='pdbsite=AC4:Ca Binding Site For Chain B'>AC4</scene>, <scene name='pdbsite=AC5:Ca Binding Site For Chain B'>AC5</scene>, <scene name='pdbsite=AC6:Ca Binding Site For Chain B'>AC6</scene> and <scene name='pdbsite=AC7:Ca Binding Site For Chain B'>AC7</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VB6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The post-rigor structure of myosin VI and implications for the recovery stroke., Menetrey J, Llinas P, Cicolari J, Squires G, Liu X, Li A, Sweeney HL, Houdusse A, EMBO J. 2007 Nov 29;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18046460 18046460]
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</div>
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<div class="pdbe-citations 2vb6" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Calmodulin 3D structures|Calmodulin 3D structures]]
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*[[Myosin 3D Structures|Myosin 3D Structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Cicolari, J.]]
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[[Category: Cicolari J]]
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[[Category: Houdusse, A.]]
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[[Category: Houdusse A]]
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[[Category: Li, A.]]
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[[Category: Li A]]
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[[Category: Liu, X.]]
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[[Category: Liu X]]
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[[Category: Llinas, P.]]
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[[Category: Llinas P]]
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[[Category: Menetrey, J.]]
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[[Category: Menetrey J]]
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[[Category: Squires, G.]]
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[[Category: Squires G]]
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[[Category: Sweeney, H.L.]]
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[[Category: Sweeney HL]]
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[[Category: ADP]]
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[[Category: BEF]]
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[[Category: CA]]
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[[Category: MG]]
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[[Category: calmodulin]]
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[[Category: cam]]
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[[Category: mg.adp.befx]]
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[[Category: molecular motor]]
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[[Category: motor protein]]
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[[Category: myosin vi]]
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[[Category: post-rigor state]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:46:46 2008''
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Current revision

Myosin VI (MD-insert2-CaM, Delta Insert1) Post-rigor state (crystal form 2)

PDB ID 2vb6

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