3n9g

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[[Image:3n9g.jpg|left|200px]]
 
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==Crystal structure of the Fab fragment of the human neutralizing anti-West Nile Virus MAb CR4354==
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The line below this paragraph, containing "STRUCTURE_3n9g", creates the "Structure Box" on the page.
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<StructureSection load='3n9g' size='340' side='right'caption='[[3n9g]], [[Resolution|resolution]] 1.43&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3n9g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N9G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.434&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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{{STRUCTURE_3n9g| PDB=3n9g | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n9g OCA], [https://pdbe.org/3n9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n9g RCSB], [https://www.ebi.ac.uk/pdbsum/3n9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n9g ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many flaviviruses are significant human pathogens, with the humoral immune response playing an essential role in restricting infection and disease. CR4354, a human monoclonal antibody isolated from a patient, neutralizes West Nile virus (WNV) infection at a postattachment stage in the viral life-cycle. Here, we determined the structure of WNV complexed with Fab fragments of CR4354 using cryoelectron microscopy. The outer glycoprotein shell of a mature WNV particle is formed by 30 rafts of three homodimers of the viral surface protein E. CR4354 binds to a discontinuous epitope formed by protein segments from two neighboring E molecules, but does not cause any detectable structural disturbance on the viral surface. The epitope occurs at two independent positions within an icosahedral asymmetric unit, resulting in 120 binding sites on the viral surface. The cross-linking of the six E monomers within one raft by four CR4354 Fab fragments suggests that the antibody neutralizes WNV by blocking the pH-induced rearrangement of the E protein required for virus fusion with the endosomal membrane.
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===Crystal structure of the Fab fragment of the human neutralizing anti-West Nile Virus MAb CR4354===
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Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354.,Kaufmann B, Vogt MR, Goudsmit J, Holdaway HA, Aksyuk AA, Chipman PR, Kuhn RJ, Diamond MS, Rossmann MG Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18950-5. Epub 2010 Oct 18. PMID:20956322<ref>PMID:20956322</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3n9g" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20956322 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20956322}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3N9G is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N9G OCA].
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==Reference==
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<ref group="xtra">PMID:20956322</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Kaufmann, B.]]
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[[Category: Large Structures]]
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[[Category: Rossmann, M G.]]
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[[Category: Kaufmann B]]
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[[Category: Anti-west nile virus]]
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[[Category: Rossmann MG]]
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[[Category: Fab fragment]]
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[[Category: Human neutralizing antibody]]
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[[Category: Immune system]]
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[[Category: Mab cr4354]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 24 14:16:30 2010''
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Current revision

Crystal structure of the Fab fragment of the human neutralizing anti-West Nile Virus MAb CR4354

PDB ID 3n9g

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