3b4b

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(New page: 200px<br /><applet load="3b4b" size="350" color="white" frame="true" align="right" spinBox="true" caption="3b4b, resolution 2.7&Aring;" /> '''T. tengcongensis glmS...)
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[[Image:3b4b.gif|left|200px]]<br /><applet load="3b4b" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="3b4b, resolution 2.7&Aring;" />
 
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'''T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage'''<br />
 
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==Overview==
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==T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage==
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The glmS ribozyme is a catalytic riboswitch that is activated for, endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using, kinetic assays and X-ray crystallography, we identify an active-site, mutation of a conserved guanine that abolishes catalysis without, perturbing coenzyme binding. Our results provide evidence that coenzyme, function requires a specific nucleobase to interact with the nucleophile, of the cleavage reaction.
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<StructureSection load='3b4b' size='340' side='right'caption='[[3b4b]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3b4b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B4B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B4B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3AD:3-DEOXYADENOSINE'>3AD</scene>, <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b4b OCA], [https://pdbe.org/3b4b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b4b RCSB], [https://www.ebi.ac.uk/pdbsum/3b4b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b4b ProSAT]</span></td></tr>
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</table>
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==About this Structure==
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==See Also==
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3B4B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis] with <scene name='pdbligand=GLP:'>GLP</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=3AD:'>3AD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B4B OCA].
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Essential role of an active-site guanine in glmS ribozyme catalysis., Klein DJ, Been MD, Ferre-D'Amare AR, J Am Chem Soc. 2007 Dec 5;129(48):14858-9. Epub 2007 Nov 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17990888 17990888]
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Thermoanaerobacter tengcongensis]]
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[[Category: Ferre-D'Amare AR]]
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[[Category: Amare, A.R.Ferre-D.]]
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[[Category: Klein DJ]]
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[[Category: Klein, D.J.]]
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[[Category: 3AD]]
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[[Category: GLP]]
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[[Category: MG]]
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[[Category: glucosamine-6-phosphate]]
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[[Category: riboswitch]]
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[[Category: ribozyme]]
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[[Category: rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:47:24 2008''
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Current revision

T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage

PDB ID 3b4b

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