3p62

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (17:02, 1 November 2023) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3p62.jpg|left|200px]]
 
-
<!--
+
==Wild-type pentaerythritol tetranitrate reductase containing a C-terminal 8-histidine tag==
-
The line below this paragraph, containing "STRUCTURE_3p62", creates the "Structure Box" on the page.
+
<StructureSection load='3p62' size='340' side='right'caption='[[3p62]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3p62]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P62 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-
{{STRUCTURE_3p62| PDB=3p62 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p62 OCA], [https://pdbe.org/3p62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p62 RCSB], [https://www.ebi.ac.uk/pdbsum/3p62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p62 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/P71278_ENTCL P71278_ENTCL]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
This work describes the development of an automated robotic platform for the rapid screening of enzyme variants generated from directed evolution studies of pentraerythritol tetranitrate (PETN) reductase, a target for industrial biocatalysis. By using a 96-well format, near pure enzyme was recovered and was suitable for high throughput kinetic assays; this enabled rapid screening for improved and new activities from libraries of enzyme variants. Initial characterisation of several single site-saturation libraries targeted at active site residues of PETN reductase, are described. Two mutants (T26S and W102F) were shown to have switched in substrate enantiopreference against substrates (E)-2-aryl-1-nitropropene and alpha-methyl-trans-cinnamaldehyde, respectively, with an increase in ee (62 % (R) for W102F). In addition, the detection of mutants with weak activity against alpha,beta-unsaturated carboxylic acid substrates showed progress in the expansion of the substrate range of PETN reductase. These methods can readily be adapted for rapid evolution of enzyme variants with other oxidoreductase enzymes.
-
===Wild-type pentaerythritol tetranitrate reductase containing a C-terminal 8-histidine tag===
+
Focused directed evolution of pentaerythritol tetranitrate reductase by using automated anaerobic kinetic screening of site-saturated libraries.,Hulley ME, Toogood HS, Fryszkowska A, Mansell D, Stephens GM, Gardiner JM, Scrutton NS Chembiochem. 2010 Nov 22;11(17):2433-47. PMID:21064170<ref>PMID:21064170</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3p62" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_21064170}}, adds the Publication Abstract to the page
+
*[[Pentaerythritol tetranitrate reductase|Pentaerythritol tetranitrate reductase]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 21064170 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_21064170}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
3P62 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P62 OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:21064170</ref><references group="xtra"/>
+
[[Category: Enterobacter cloacae]]
[[Category: Enterobacter cloacae]]
-
[[Category: NADPH dehydrogenase]]
+
[[Category: Large Structures]]
-
[[Category: Hulley, M E.]]
+
[[Category: Hulley ME]]
-
[[Category: Scrutton, N S.]]
+
[[Category: Scrutton NS]]
-
[[Category: Toogood, H S.]]
+
[[Category: Toogood HS]]
-
[[Category: Alkene reduction]]
+
-
[[Category: Alpha]]
+
-
[[Category: Beta barrel]]
+
-
[[Category: Old yellow enzyme family]]
+
-
[[Category: Oxidoreductase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 24 14:20:03 2010''
+

Current revision

Wild-type pentaerythritol tetranitrate reductase containing a C-terminal 8-histidine tag

PDB ID 3p62

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3p62"
Personal tools