7ins

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[[Image:7ins.gif|left|200px]]<br /><applet load="7ins" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="7ins, resolution 2.0&Aring;" />
 
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'''STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z'''<br />
 
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==Overview==
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==STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z==
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The crystal structure of NPH-insulin, pig insulin cocrystallized with, zinc, m-cresol and protamine, has been solved by molecular replacement and, refined using restrained least-squares refinement methods. The final, crystallographic R factor for all reflections between 2 and 10 A is 19.4%., The insulin molecules are arranged as hexamers with two tetrahedrally, coordinated Zn atoms in the central channel and one m-cresol bound to each, monomer near His B5. One protamine binding site has been unequivocally, identified near a dimer-dimer interface, although most of the polypeptide, is crystallographically disordered. The conformation of the insulin moiety, and the structural differences between the three unique monomers have been, analysed. The zinc and m-cresol environments are described and the nature, of the protamine binding site is outlined.
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<StructureSection load='7ins' size='340' side='right'caption='[[7ins]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7ins]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7INS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7INS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ins FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ins OCA], [https://pdbe.org/7ins PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ins RCSB], [https://www.ebi.ac.uk/pdbsum/7ins PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ins ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/INS_PIG INS_PIG] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/in/7ins_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=7ins ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of NPH-insulin, pig insulin cocrystallized with zinc, m-cresol and protamine, has been solved by molecular replacement and refined using restrained least-squares refinement methods. The final crystallographic R factor for all reflections between 2 and 10 A is 19.4%. The insulin molecules are arranged as hexamers with two tetrahedrally coordinated Zn atoms in the central channel and one m-cresol bound to each monomer near His B5. One protamine binding site has been unequivocally identified near a dimer-dimer interface, although most of the polypeptide is crystallographically disordered. The conformation of the insulin moiety and the structural differences between the three unique monomers have been analysed. The zinc and m-cresol environments are described and the nature of the protamine binding site is outlined.
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==About this Structure==
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Structure of porcine insulin cocrystallized with clupeine Z.,Balschmidt P, Hansen FB, Dodson EJ, Dodson GG, Korber F Acta Crystallogr B. 1991 Dec 1;47 ( Pt 6):975-86. PMID:1772633<ref>PMID:1772633</ref>
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7INS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=UNK:'>UNK</scene> and <scene name='pdbligand=CRS:'>CRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=MCR:M-Cresol Binding Site. Oh Binds On Each Monomer To Oa6 A ...'>MCR</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7INS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of porcine insulin cocrystallized with clupeine Z., Balschmidt P, Hansen FB, Dodson EJ, Dodson GG, Korber F, Acta Crystallogr B. 1991 Dec 1;47 ( Pt 6):975-86. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1772633 1772633]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 7ins" style="background-color:#fffaf0;"></div>
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[[Category: Sus scrofa]]
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[[Category: Balschmidt, P.]]
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[[Category: Dodson, E.]]
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[[Category: Dodson, G.]]
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[[Category: Hansen, F.B.]]
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[[Category: Korber, F.]]
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[[Category: CRS]]
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[[Category: UNK]]
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[[Category: ZN]]
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[[Category: hormone]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:50:45 2008''
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==See Also==
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*[[Insulin 3D Structures|Insulin 3D Structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Sus scrofa]]
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[[Category: Balschmidt P]]
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[[Category: Dodson E]]
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[[Category: Dodson G]]
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[[Category: Hansen FB]]
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[[Category: Korber F]]

Current revision

STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z

PDB ID 7ins

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