2jpz

[[Image:2jpz.gif|left|200px]]<br /><applet load="2jpz" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2jpz" />

'''Structure of the hybrid-2 type intramolecular human telomeric G-quadruplex'''<br />

==Overview==

Formation of the G-quadruplex in the human telomeric sequence can inhibit, the activity of telomerase, thus the intramolecular telomeric, G-quadruplexes have been considered as an attractive anticancer target., Information of intramolecular telomeric G-quadruplex structures formed, under physiological conditions is important for structure-based drug, design. Here, we report the first structure of the major intramolecular, G-quadruplex formed in a native, non-modified human telomeric sequence in, K(+) solution. This is a hybrid-type mixed, parallel/antiparallel-G-stranded G-quadruplex, one end of which is covered, by a novel T:A:T triple capping structure. This structure (Hybrid-2) and, the previously reported Hybrid-1 structure differ in their loop, arrangements, strand orientations and capping structures. The distinct, capping structures appear to be crucial for the favored formation of the, specific hybrid-type intramolecular telomeric G-quadruplexes, and may, provide specific binding sites for drug targeting. Our study also shows, that while the hybrid-type G-quadruplexes appear to be the major, conformations in K(+) solution, human telomeric sequences are always in, equilibrium between Hybrid-1 and Hybrid-2 structures, which is largely, determined by the 3'-flanking sequence. Furthermore, both hybrid-type, G-quadruplexes suggest a straightforward means for multimer formation with, effective packing in the human telomeric sequence and provide important, implications for drug targeting of G-quadruplexes in human telomeres.

 

==About this Structure==

2JPZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JPZ OCA].

 

Structure of the Hybrid-2 type intramolecular human telomeric G-quadruplex in K+ solution: insights into structure polymorphism of the human telomeric sequence., Dai J, Carver M, Punchihewa C, Jones RA, Yang D, Nucleic Acids Res. 2007;35(15):4927-40. Epub 2007 Jul 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17626043 17626043]

[[Category: Protein complex]]

[[Category: Carver, M.]]

[[Category: Dai, J.]]

[[Category: Jones, R.]]

[[Category: Punchihewa, C.]]

[[Category: Yang, D.]]