2qzv

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(New page: 200px<br /><applet load="2qzv" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qzv, resolution 9.00&Aring;" /> '''Draft Crystal Struct...)
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[[Image:2qzv.jpg|left|200px]]<br /><applet load="2qzv" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qzv, resolution 9.00&Aring;" />
 
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'''Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution'''<br />
 
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==Overview==
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==Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution==
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The vault is a highly conserved ribonucleoprotein particle found in all, higher eukaryotes. It has a barrel-shaped structure and is composed of the, major vault protein (MVP); vault poly(ADP-ribose) polymerase (VPARP);, telomerase-associated protein 1 (TEP1); and small untranslated RNA (vRNA)., Although its strong conservation and high abundance indicate an important, cellular role, the function of the vault is unknown. In humans, vaults, have been implicated in multidrug resistance during chemotherapy., Recently, assembly of recombinant vaults has been established in insect, cells expressing only MVP. Here, we demonstrate that co-expression of MVP, with one or both of the other two vault proteins results in their, co-assembly into regularly shaped vaults. Particles assembled from MVP, with N-terminal peptide tags of various length are compared. Cryoelectron, microscopy (cryoEM) and single-particle image reconstruction methods were, used to determine the structure of nine recombinant vaults of various, composition, as well as wild-type and TEP1-deficient mouse vaults., Recombinant vaults with MVP N-terminal peptide tags showed internal, density that varied in size with the length of the tag. Reconstruction of, a recombinant vault with a cysteine-rich tag revealed 48-fold rotational, symmetry for the vault. A model is proposed for the organization of MVP, within the vault with all of the MVP N termini interacting non-covalently, at the vault midsection and 48 copies of MVP forming each half vault., CryoEM difference mapping localized VPARP to three density bands lining, the inner surface of the vault. Difference maps designed to localize TEP1, showed only weak density inside of the caps, suggesting that TEP1 may, interact with MVP via a small interaction region. In the absence of, atomic-resolution structures for either VPARP or TEP1, fold recognition, methods were applied. A total of 21 repeats were predicted for the TEP1, WD-repeat domain, suggesting an unusually large beta-propeller fold.
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<StructureSection load='2qzv' size='340' side='right'caption='[[2qzv]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2qzv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QZV FirstGlance]. <br>
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2QZV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QZV OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qzv OCA], [https://pdbe.org/2qzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qzv RCSB], [https://www.ebi.ac.uk/pdbsum/2qzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qzv ProSAT]</span></td></tr>
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==Reference==
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</table>
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Cryoelectron microscopy imaging of recombinant and tissue derived vaults: localization of the MVP N termini and VPARP., Mikyas Y, Makabi M, Raval-Fernandes S, Harrington L, Kickhoefer VA, Rome LH, Stewart PL, J Mol Biol. 2004 Nov 12;344(1):91-105. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15504404 15504404]
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== Function ==
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[https://www.uniprot.org/uniprot/MVP_RAT MVP_RAT] Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/2qzv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qzv ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Anderson DH]]
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[[Category: Anderson, D.H.]]
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[[Category: Eisenberg D]]
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[[Category: Eisenberg, D.]]
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[[Category: Kickhoefer VA]]
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[[Category: Kickhoefer, V.A.]]
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[[Category: Rome LH]]
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[[Category: Rome, L.H.]]
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[[Category: Sievers SA]]
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[[Category: Sievers, S.A.]]
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[[Category: barrel self-assembled from 96 staves]]
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[[Category: cytoplasm]]
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[[Category: innate immunity]]
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[[Category: major vault protein]]
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[[Category: nanocapsule]]
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[[Category: ribonucleoprotein]]
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[[Category: structural protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:52:15 2008''
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Current revision

Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution

PDB ID 2qzv

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