3fpf

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{{Seed}}
 
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[[Image:3fpf.png|left|200px]]
 
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==Crystal Structure of MtNAS in complex with MTA and tNA==
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The line below this paragraph, containing "STRUCTURE_3fpf", creates the "Structure Box" on the page.
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<StructureSection load='3fpf' size='340' side='right'caption='[[3fpf]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3fpf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._Delta_H Methanothermobacter thermautotrophicus str. Delta H]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3FPF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene>, <scene name='pdbligand=TNA:N-[(3S)-3-{[(3S)-3-AMINO-3-CARBOXYPROPYL]AMINO}-3-CARBOXYPROPYL]-L-GLUTAMIC+ACID'>TNA</scene></td></tr>
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{{STRUCTURE_3fpf| PDB=3fpf | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3fpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fpf OCA], [https://pdbe.org/3fpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3fpf RCSB], [https://www.ebi.ac.uk/pdbsum/3fpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3fpf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O26771_METTH O26771_METTH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/3fpf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3fpf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nicotianamine (NA), a small molecule ubiquitous in plants, is an important divalent metal chelator and the main precursor of phytosiderophores. Nicotianamine synthase (NAS) is the enzyme catalyzing NA synthesis by the condensation of three aminopropyl moieties of S-adenosylmethionine (SAM) and the cyclization of one of them to form an azetidine ring. Here we report five crystal structures of an archaeal NAS from Methanothermobacter thermautotrophicus, either free or in complex with its product(s) and substrate(s). These structures reveal a two-domains fold arrangement of MtNAS, a small molecule related to NA (named here thermoNicotianamine or tNA), and an original mechanism of synthesis in a buried reaction chamber. This reaction chamber is open to the solvent through a small inlet, and a single active site allows the selective entrance of only one substrate at a time that is then processed and translocated stepwise.
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===Crystal Structure of MtNAS in complex with MTA and tNA===
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Crystallographic snapshots of iterative substrate translocations during nicotianamine synthesis in Archaea.,Dreyfus C, Lemaire D, Mari S, Pignol D, Arnoux P Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16180-4. Epub 2009 Sep 2. PMID:19805277<ref>PMID:19805277</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19805277}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3fpf" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19805277 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19805277}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3FPF is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FPF OCA].
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[[Category: Methanothermobacter thermautotrophicus str. Delta H]]
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[[Category: Arnoux P]]
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==Reference==
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[[Category: Dreyfus C]]
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<ref group="xtra">PMID:19805277</ref><references group="xtra"/>
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[[Category: Pignol D]]
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[[Category: Methanothermobacter thermautotrophicus]]
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[[Category: Arnoux, P.]]
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[[Category: Dreyfus, C.]]
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[[Category: Pignol, D.]]
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[[Category: Biosynthetic protein]]
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[[Category: Nicotianamine]]
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[[Category: Thermonicotianamine]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 8 11:19:01 2010''
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Current revision

Crystal Structure of MtNAS in complex with MTA and tNA

PDB ID 3fpf

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