3psz
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3psz is ON HOLD Authors: Xiong, X., Bromley, E.H.C., Oelschlaeger, P., Woolfson, D.N., Spencer, J. Description: Crystal Structure of AhQnr, the Qnr...) |
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- | '''Unreleased structure''' | ||
- | + | ==Crystal Structure of AhQnr, the Qnr protein from Aeromonas hydrophila (P21212 crystal form)== | |
+ | <StructureSection load='3psz' size='340' side='right'caption='[[3psz]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[3psz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_hydrophila_subsp._hydrophila_ATCC_7966 Aeromonas hydrophila subsp. hydrophila ATCC 7966]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PSZ FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | ||
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CAS:S-(DIMETHYLARSENIC)CYSTEINE'>CAS</scene>, <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3psz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3psz OCA], [https://pdbe.org/3psz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3psz RCSB], [https://www.ebi.ac.uk/pdbsum/3psz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3psz ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/A0KF03_AERHH A0KF03_AERHH] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Quinolones inhibit bacterial type II DNA topoisomerases (e.g. DNA gyrase) and are among the most important antibiotics in current use. However, their efficacy is now being threatened by various plasmid-mediated resistance determinants. Of these, the pentapeptide repeat-containing (PRP) Qnr proteins are believed to act as DNA mimics and are particularly prevalent in Gram-negative bacteria. Predicted Qnr-like proteins are also present in numerous environmental bacteria. Here, we demonstrate that one such, Aeromonas hydrophila AhQnr, is soluble, stable, and relieves quinolone inhibition of Escherichia coli DNA gyrase, thus providing an appropriate model system for Gram-negative Qnr proteins. The AhQnr crystal structure, the first for any Gram-negative Qnr, reveals two prominent loops (1 and 2) that project from the PRP structure. Deletion mutagenesis demonstrates that both contribute to protection of E. coli DNA gyrase from quinolones. Sequence comparisons indicate that these are likely to be present across the full range of Gram-negative Qnr proteins. On this basis we present a model for the AhQnr:DNA gyrase interaction where loop1 interacts with the gyrase A 'tower' and loop2 with the gyrase B TOPRIM domains. We propose this to be a general mechanism directing the interactions of Qnr proteins with DNA gyrase in Gram-negative bacteria. | ||
- | + | Structural insights into quinolone antibiotic resistance mediated by pentapeptide repeat proteins: conserved surface loops direct the activity of a Qnr protein from a Gram-negative bacterium.,Xiong X, Bromley EH, Oelschlaeger P, Woolfson DN, Spencer J Nucleic Acids Res. 2011 Jan 11. PMID:21227918<ref>PMID:21227918</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | + | </div> | |
- | + | <div class="pdbe-citations 3psz" style="background-color:#fffaf0;"></div> | |
+ | == References == | ||
+ | <references/> | ||
+ | __TOC__ | ||
+ | </StructureSection> | ||
+ | [[Category: Aeromonas hydrophila subsp. hydrophila ATCC 7966]] | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Spencer J]] | ||
+ | [[Category: Xiong X]] |
Current revision
Crystal Structure of AhQnr, the Qnr protein from Aeromonas hydrophila (P21212 crystal form)
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