3o3g

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{{Seed}}
 
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[[Image:3o3g.jpg|left|200px]]
 
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==T. maritima RNase H2 in complex with nucleic acid substrate and calcium ions==
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The line below this paragraph, containing "STRUCTURE_3o3g", creates the "Structure Box" on the page.
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<StructureSection load='3o3g' size='340' side='right'caption='[[3o3g]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3o3g]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O3G FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_3o3g| PDB=3o3g | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3g OCA], [https://pdbe.org/3o3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o3g RCSB], [https://www.ebi.ac.uk/pdbsum/3o3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o3g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNH2_THEMA RNH2_THEMA] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Two classes of RNase H hydrolyze RNA of RNA/DNA hybrids. In contrast to RNase H1 that requires four ribonucleotides for cleavage, RNase H2 can nick duplex DNAs containing a single ribonucleotide, suggesting different in vivo substrates. We report here the crystal structures of a type 2 RNase H in complex with substrates containing a (5')RNA-DNA(3') junction. They revealed a unique mechanism of recognition and substrate-assisted cleavage. A conserved tyrosine residue distorts the nucleic acid at the junction, allowing the substrate to function in catalysis by participating in coordination of the active site metal ion. The biochemical and structural properties of RNase H2 explain the preference of the enzyme for junction substrates and establish the structural and mechanistic differences with RNase H1. Junction recognition is important for the removal of RNA embedded in DNA and may play an important role in DNA replication and repair.
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===T. maritima RNase H2 in complex with nucleic acid substrate and calcium ions===
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Crystal Structures of RNase H2 in Complex with Nucleic Acid Reveal the Mechanism of RNA-DNA Junction Recognition and Cleavage.,Rychlik MP, Chon H, Cerritelli SM, Klimek P, Crouch RJ, Nowotny M Mol Cell. 2010 Nov 24;40(4):658-70. PMID:21095591<ref>PMID:21095591</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3o3g" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21095591}}, adds the Publication Abstract to the page
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21095591 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21095591}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3O3G is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3G OCA].
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==Reference==
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<ref group="xtra">PMID:21095591</ref><references group="xtra"/>
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[[Category: Ribonuclease H]]
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[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Cerritelli, S M.]]
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[[Category: Cerritelli SM]]
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[[Category: Chon, H.]]
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[[Category: Chon H]]
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[[Category: Crouch, R J.]]
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[[Category: Crouch RJ]]
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[[Category: Klimek, P.]]
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[[Category: Klimek P]]
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[[Category: Nowotny, M.]]
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[[Category: Nowotny M]]
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[[Category: Rychlik, M P.]]
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[[Category: Rychlik MP]]
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[[Category: Hydrolase-dna-rna hybrid complex]]
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[[Category: Nuclease]]
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[[Category: Rnase h fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 8 11:51:34 2010''
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Current revision

T. maritima RNase H2 in complex with nucleic acid substrate and calcium ions

PDB ID 3o3g

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