2j59

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[[Image:2j59.gif|left|200px]]<br /><applet load="2j59" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2j59, resolution 2.1&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE ARF1:ARHGAP21-ARFBD COMPLEX'''<br />
 
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==Overview==
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==Crystal structure of the ARF1:ARHGAP21-ArfBD complex==
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ARHGAP21 is a Rho family GTPase-activating protein (RhoGAP) that controls, the Arp2/3 complex and F-actin dynamics at the Golgi complex by regulating, the activity of the small GTPase Cdc42. ARHGAP21 is recruited to the Golgi, by binding to another small GTPase, ARF1. Here, we present the crystal, structure of the activated GTP-bound form of ARF1 in a complex with the, Arf-binding domain (ArfBD) of ARHGAP21 at 2.1 A resolution. We show that, ArfBD comprises a PH domain adjoining a C-terminal alpha helix, and that, ARF1 interacts with both of these structural motifs through its switch, regions and triggers structural rearrangement of the PH domain. We used, site-directed mutagenesis to confirm that both the PH domain and the, helical motif are essential for the binding of ArfBD to ARF1 and for its, recruitment to the Golgi. Our data demonstrate that two well-known small, GTPase-binding motifs, the PH domain and the alpha helical motif, can, combine to create a novel mode of binding to Arfs.
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<StructureSection load='2j59' size='340' side='right'caption='[[2j59]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2j59]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J59 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j59 OCA], [https://pdbe.org/2j59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j59 RCSB], [https://www.ebi.ac.uk/pdbsum/2j59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j59 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARF1_MOUSE ARF1_MOUSE] GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j5/2j59_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j59 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ARHGAP21 is a Rho family GTPase-activating protein (RhoGAP) that controls the Arp2/3 complex and F-actin dynamics at the Golgi complex by regulating the activity of the small GTPase Cdc42. ARHGAP21 is recruited to the Golgi by binding to another small GTPase, ARF1. Here, we present the crystal structure of the activated GTP-bound form of ARF1 in a complex with the Arf-binding domain (ArfBD) of ARHGAP21 at 2.1 A resolution. We show that ArfBD comprises a PH domain adjoining a C-terminal alpha helix, and that ARF1 interacts with both of these structural motifs through its switch regions and triggers structural rearrangement of the PH domain. We used site-directed mutagenesis to confirm that both the PH domain and the helical motif are essential for the binding of ArfBD to ARF1 and for its recruitment to the Golgi. Our data demonstrate that two well-known small GTPase-binding motifs, the PH domain and the alpha helical motif, can combine to create a novel mode of binding to Arfs.
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==About this Structure==
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Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes.,Menetrey J, Perderiset M, Cicolari J, Dubois T, Elkhatib N, El Khadali F, Franco M, Chavrier P, Houdusse A EMBO J. 2007 Apr 4;26(7):1953-62. Epub 2007 Mar 8. PMID:17347647<ref>PMID:17347647</ref>
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2J59 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=GTP:'>GTP</scene>, <scene name='pdbligand=DIO:'>DIO</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Gtp Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Mg Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Gtp Binding Site For Chain B'>AC3</scene>, <scene name='pdbsite=AC4:Mg Binding Site For Chain B'>AC4</scene>, <scene name='pdbsite=AC5:Gtp Binding Site For Chain C'>AC5</scene>, <scene name='pdbsite=AC6:Mg Binding Site For Chain C'>AC6</scene>, <scene name='pdbsite=AC7:Gtp Binding Site For Chain D'>AC7</scene>, <scene name='pdbsite=AC8:Mg Binding Site For Chain D'>AC8</scene>, <scene name='pdbsite=AC9:Gtp Binding Site For Chain E'>AC9</scene>, <scene name='pdbsite=BC1:Mg Binding Site For Chain E'>BC1</scene>, <scene name='pdbsite=BC2:Gtp Binding Site For Chain F'>BC2</scene>, <scene name='pdbsite=BC3:Mg Binding Site For Chain F'>BC3</scene>, <scene name='pdbsite=BC4:So4 Binding Site For Chain M'>BC4</scene>, <scene name='pdbsite=BC5:So4 Binding Site For Chain N'>BC5</scene>, <scene name='pdbsite=BC6:So4 Binding Site For Chain O'>BC6</scene>, <scene name='pdbsite=BC7:So4 Binding Site For Chain P'>BC7</scene>, <scene name='pdbsite=BC8:So4 Binding Site For Chain Q'>BC8</scene>, <scene name='pdbsite=BC9:So4 Binding Site For Chain R'>BC9</scene>, <scene name='pdbsite=CC1:Dio Binding Site For Chain A'>CC1</scene>, <scene name='pdbsite=CC2:Dio Binding Site For Chain B'>CC2</scene>, <scene name='pdbsite=CC3:Dio Binding Site For Chain F'>CC3</scene>, <scene name='pdbsite=CC4:Edo Binding Site For Chain C'>CC4</scene>, <scene name='pdbsite=CC5:Edo Binding Site For Chain O'>CC5</scene> and <scene name='pdbsite=CC6:Edo Binding Site For Chain E'>CC6</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J59 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes., Menetrey J, Perderiset M, Cicolari J, Dubois T, Elkhatib N, El Khadali F, Franco M, Chavrier P, Houdusse A, EMBO J. 2007 Apr 4;26(7):1953-62. Epub 2007 Mar 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17347647 17347647]
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</div>
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<div class="pdbe-citations 2j59" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Rho GTPase activating protein 3D structures|Rho GTPase activating protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Chavrier P]]
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[[Category: Chavrier, P.]]
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[[Category: Cicolari J]]
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[[Category: Cicolari, J.]]
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[[Category: Dubois T]]
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[[Category: Dubois, T.]]
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[[Category: El Khadali F]]
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[[Category: Franco, M.]]
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[[Category: El Khatib N]]
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[[Category: Houdusse, A.]]
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[[Category: Franco M]]
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[[Category: Khadali, F.El.]]
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[[Category: Houdusse A]]
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[[Category: Khatib, N.El.]]
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[[Category: Menetrey J]]
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[[Category: Menetrey, J.]]
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[[Category: Perderiset M]]
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[[Category: Perderiset, M.]]
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[[Category: DIO]]
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[[Category: EDO]]
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[[Category: GTP]]
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[[Category: MG]]
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[[Category: SO4]]
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[[Category: actin organization]]
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[[Category: arf]]
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[[Category: arf1]]
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[[Category: arfbd]]
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[[Category: arhgap21]]
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[[Category: golgi apparatus]]
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[[Category: hydrolase]]
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[[Category: myristate]]
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[[Category: nucleotide-binding]]
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[[Category: protein transport]]
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[[Category: rhogap protein]]
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[[Category: small gtp-binding protein]]
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[[Category: transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:02:21 2008''
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Current revision

Crystal structure of the ARF1:ARHGAP21-ArfBD complex

PDB ID 2j59

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