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2nts

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(New page: 200px<br /><applet load="2nts" size="350" color="white" frame="true" align="right" spinBox="true" caption="2nts, resolution 2.400&Aring;" /> '''Crystal Structure o...)
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[[Image:2nts.gif|left|200px]]<br /><applet load="2nts" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2nts, resolution 2.400&Aring;" />
 
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'''Crystal Structure of SEK-hVb5.1'''<br />
 
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==Overview==
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==Crystal Structure of SEK-hVb5.1==
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Superantigens (SAGs) interact with host immune receptors to induce a, massive release of inflammatory cytokines that can lead to toxic shock, syndrome and death. Bacterial SAGs can be classified into five distinct, evolutionary groups. Group V SAGs are characterized by the alpha3-beta8, loop, a unique approximately 15 amino acid residue extension that is, required for optimal T cell activation. Here, we report the X-ray crystal, structures of the group V SAG staphylococcal enterotoxin K (SEK) alone and, in complex with the TCR hVbeta5.1 domain. SEK adopts a unique TCR binding, orientation relative to other SAG-TCR complexes, which results in the, alpha3-beta8 loop contacting the apical loop of framework region 4, thereby extending the known TCR recognition site of SAGs. These, interactions are absolutely required for TCR binding and T cell activation, by SEK, and dictate the TCR Vbeta domain specificity of SEK and other, group V SAGs.
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<StructureSection load='2nts' size='340' side='right'caption='[[2nts]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nts]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_COL Staphylococcus aureus subsp. aureus COL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NTS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nts OCA], [https://pdbe.org/2nts PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nts RCSB], [https://www.ebi.ac.uk/pdbsum/2nts PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nts ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRBC1_HUMAN TRBC1_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/2nts_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nts ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Superantigens (SAGs) interact with host immune receptors to induce a massive release of inflammatory cytokines that can lead to toxic shock syndrome and death. Bacterial SAGs can be classified into five distinct evolutionary groups. Group V SAGs are characterized by the alpha3-beta8 loop, a unique approximately 15 amino acid residue extension that is required for optimal T cell activation. Here, we report the X-ray crystal structures of the group V SAG staphylococcal enterotoxin K (SEK) alone and in complex with the TCR hVbeta5.1 domain. SEK adopts a unique TCR binding orientation relative to other SAG-TCR complexes, which results in the alpha3-beta8 loop contacting the apical loop of framework region 4, thereby extending the known TCR recognition site of SAGs. These interactions are absolutely required for TCR binding and T cell activation by SEK, and dictate the TCR Vbeta domain specificity of SEK and other group V SAGs.
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==About this Structure==
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A novel loop domain in superantigens extends their T cell receptor recognition site.,Gunther S, Varma AK, Moza B, Kasper KJ, Wyatt AW, Zhu P, Rahman AK, Li Y, Mariuzza RA, McCormick JK, Sundberg EJ J Mol Biol. 2007 Aug 3;371(1):210-21. Epub 2007 May 18. PMID:17560605<ref>PMID:17560605</ref>
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2NTS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A Novel Loop Domain in Superantigens Extends their T Cell Receptor Recognition Site., Gunther S, Varma AK, Moza B, Kasper KJ, Wyatt AW, Zhu P, Rahman AK, Li Y, Mariuzza RA, McCormick JK, Sundberg EJ, J Mol Biol. 2007 May 18;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17560605 17560605]
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</div>
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<div class="pdbe-citations 2nts" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Staphylococcus aureus]]
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[[Category: Staphylococcus aureus subsp. aureus COL]]
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[[Category: Gunther, S.]]
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[[Category: Gunther S]]
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[[Category: Moza, B.]]
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[[Category: Moza B]]
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[[Category: Sundberg, E.J.]]
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[[Category: Sundberg EJ]]
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[[Category: Varma, A.K.]]
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[[Category: Varma AK]]
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[[Category: superantigen; t cell receptor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:02:34 2008''
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Current revision

Crystal Structure of SEK-hVb5.1

PDB ID 2nts

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