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2r4g

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(New page: 200px<br /><applet load="2r4g" size="350" color="white" frame="true" align="right" spinBox="true" caption="2r4g, resolution 1.71&Aring;" /> '''The high resolution ...)
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[[Image:2r4g.jpg|left|200px]]<br /><applet load="2r4g" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2r4g, resolution 1.71&Aring;" />
 
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'''The high resolution structure of the RNA-binding domain of telomerase'''<br />
 
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==Overview==
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==The high resolution structure of the RNA-binding domain of telomerase==
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Telomerase, a ribonucleoprotein complex, replicates the linear ends of, eukaryotic chromosomes, thus taking care of the "end of replication, problem." TERT contains an essential and universally conserved domain, (TRBD) that makes extensive contacts with the RNA (TER) component of the, holoenzyme, and this interaction is thought to facilitate TERT/TER, assembly and repeat-addition processivity. Here, we present a, high-resolution structure of TRBD from Tetrahymena thermophila. The nearly, all-helical structure comprises a nucleic acid-binding fold suitable for, TER binding. An extended pocket on the surface of the protein, formed by, two conserved motifs (CP and T motifs) comprises TRBD's RNA-binding, pocket. The width and the chemical nature of this pocket suggest that it, binds both single- and double-stranded RNA, possibly stem I, and the, template boundary element (TBE). Moreover, the structure provides clues, into the role of this domain in TERT/TER stabilization and telomerase, repeat-addition processivity.
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<StructureSection load='2r4g' size='340' side='right'caption='[[2r4g]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2r4g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R4G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r4g OCA], [https://pdbe.org/2r4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r4g RCSB], [https://www.ebi.ac.uk/pdbsum/2r4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r4g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TERT_TETTS TERT_TETTS] Catalytic component of telomerase, an essential ribonucleoprotein enzyme that copies new telomeric repeats onto chromosome ends by repetitively synthesizing the short telomere-repeat sequence 5'-TTGGGG-3' using an RNA template component TER (PubMed:10944124, PubMed:15696174, PubMed:17322903, PubMed:20713447, PubMed:16462747). TERT is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme (PubMed:10944124, PubMed:15696174, PubMed:17322903, PubMed:20713447, PubMed:16462747).<ref>PMID:10944124</ref> <ref>PMID:15696174</ref> <ref>PMID:16462747</ref> <ref>PMID:17322903</ref> <ref>PMID:20713447</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r4/2r4g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r4g ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2R4G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila] with <scene name='pdbligand=BR:'>BR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R4G OCA].
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*[[Telomerase 3D structures|Telomerase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of the RNA-Binding Domain of Telomerase: Implications for RNA Recognition and Binding., Rouda S, Skordalakes E, Structure. 2007 Nov 13;15(11):1403-1412. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17997966 17997966]
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__TOC__
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[[Category: RNA-directed DNA polymerase]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
[[Category: Tetrahymena thermophila]]
[[Category: Tetrahymena thermophila]]
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[[Category: Rouda, S.]]
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[[Category: Rouda S]]
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[[Category: Skordalakes, E.]]
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[[Category: Skordalakes E]]
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[[Category: BR]]
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[[Category: chromosomal protein]]
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[[Category: dna-binding]]
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[[Category: nucleotidyltransferase]]
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[[Category: nucleus]]
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[[Category: rna-directed dna polymerase]]
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[[Category: telomerase]]
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[[Category: telomeres]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:06:58 2008''
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Current revision

The high resolution structure of the RNA-binding domain of telomerase

PDB ID 2r4g

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