2owy

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(New page: 200px<br /><applet load="2owy" size="350" color="white" frame="true" align="right" spinBox="true" caption="2owy, resolution 2.500&Aring;" /> '''The recombination-a...)
Current revision (13:53, 13 March 2024) (edit) (undo)
 
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[[Image:2owy.jpg|left|200px]]<br /><applet load="2owy" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2owy, resolution 2.500&Aring;" />
 
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'''The recombination-associated protein RdgC adopts a novel toroidal architecture for DNA binding'''<br />
 
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==Overview==
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==The recombination-associated protein RdgC adopts a novel toroidal architecture for DNA binding==
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RecA plays a central role in the nonmutagenic repair of stalled, replication forks in bacteria. RdgC, a recombination-associated, DNA-binding protein, is a potential negative regulator of RecA function., Here, we have determined the crystal structure of RdgC from Pseudomonas, aeruginosa. The J-shaped monomer has a unique fold and can be divided into, three structural domains: tip domain, center domain and base domain. Two, such monomers dimerize to form a ring-shaped molecule of approximate, 2-fold symmetry. Of the two inter-subunit interfaces within the dimer, one, interface ('interface A') between tip/center domains is more nonpolar than, the other ('interface B') between base domains. The structure allows us to, propose that the RdgC dimer binds dsDNA through the central hole of, approximately 30 A diameter. The proposed model is supported by our, DNA-binding assays coupled with mutagenesis, which indicate that the, conserved positively charged residues on the protein surface around the, central hole play important roles in DNA binding. The novel ring-shaped, architecture of the RdgC dimer has significant implications for its role, in homologous recombination.
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<StructureSection load='2owy' size='340' side='right'caption='[[2owy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2owy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OWY FirstGlance]. <br>
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2OWY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OWY OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2owy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2owy OCA], [https://pdbe.org/2owy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2owy RCSB], [https://www.ebi.ac.uk/pdbsum/2owy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2owy ProSAT]</span></td></tr>
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==Reference==
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</table>
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The recombination-associated protein RdgC adopts a novel toroidal architecture for DNA binding., Ha JY, Kim HK, Kim do J, Kim KH, Oh SJ, Lee HH, Yoon HJ, Song HK, Suh SW, Nucleic Acids Res. 2007;35(8):2671-81. Epub 2007 Apr 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17426134 17426134]
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== Function ==
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[[Category: Pseudomonas aeruginosa]]
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[https://www.uniprot.org/uniprot/RDGC_PSEAE RDGC_PSEAE] May be involved in recombination (By similarity).
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Suh, S.W.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: homologous recombination]]
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Check<jmol>
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[[Category: pseudomonas aeruginosa]]
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<jmolCheckbox>
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[[Category: rdgc]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ow/2owy_consurf.spt"</scriptWhenChecked>
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[[Category: reca]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: ring-shaped dna binding proteins]]
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:09:16 2008''
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2owy ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Suh SW]]

Current revision

The recombination-associated protein RdgC adopts a novel toroidal architecture for DNA binding

PDB ID 2owy

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