3llz

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{{Seed}}
 
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[[Image:3llz.png|left|200px]]
 
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==Crystal Structure Analysis of Maclura pomifera agglutinin complex with Gal-beta-1,3-GalNAc==
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The line below this paragraph, containing "STRUCTURE_3llz", creates the "Structure Box" on the page.
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<StructureSection load='3llz' size='340' side='right'caption='[[3llz]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3llz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Maclura_pomifera Maclura pomifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LLZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LLZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
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{{STRUCTURE_3llz| PDB=3llz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3llz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3llz OCA], [https://pdbe.org/3llz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3llz RCSB], [https://www.ebi.ac.uk/pdbsum/3llz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3llz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LECA_MACPO LECA_MACPO] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ll/3llz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3llz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Maclura pomifera agglutinin (MPA) recognizes the T-antigen disaccharide Galbeta1,3GalNAc mainly through interaction of the alpha-GalNAc moiety with its primary site, but the interactions of the two flanking subsites A and B with aglycones and substituents other than Gal, respectively, are not well understood. We therefore characterized the specificity of MPA in more detail by glycan micro-array analysis and determined the crystal structures of MPA without ligand and in complexes with Galbeta1,3GalNAc and p-nitrophenyl alpha-GalNAc. In both sugar complexes, pairs of ligands created inter-tetramer hydrogen-bond bridging networks. While subsite A showed increased affinity for hydrophobic aglycones, it also accommodated several sugar substituents. Notably, a GalNAc-O-tripeptide, a Tn antigen mimic, showed lower affinity than these compounds in surface plasmon resonance experiments. The glycan array data showed subsite B accepted compounds in which the O3 position of the GalNAc was substituted with various sugars other than Gal, but substitutions at O6 led to inactivity. Additions to the Gal moiety of the disaccharide also had only small effects on reactivity. These results are all compatible with the features seen in the crystal structures.
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===Crystal Structure Analysis of Maclura pomifera agglutinin complex with Gal-beta-1,3-GalNAc===
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Characterization of the secondary binding sites of Maclura pomifera agglutinin by glycan array and crystallographic analyses.,Huang J, Xu Z, Wang D, Ogata CM, Palczewski K, Lee X, Young NM Glycobiology. 2010 Sep 8. PMID:20826825<ref>PMID:20826825</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3llz" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20826825}}, adds the Publication Abstract to the page
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*[[Agglutinin 3D structures|Agglutinin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20826825 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20826825}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3LLZ is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Maclura_pomifera Maclura pomifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LLZ OCA].
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==Reference==
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<ref group="xtra">PMID:20826825</ref><references group="xtra"/>
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[[Category: Maclura pomifera]]
[[Category: Maclura pomifera]]
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[[Category: Hazen, S L.]]
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[[Category: Hazen SL]]
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[[Category: Hirama, T.]]
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[[Category: Hirama T]]
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[[Category: Huang, J.]]
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[[Category: Huang J]]
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[[Category: Lee, X.]]
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[[Category: Lee X]]
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[[Category: Ogato, C.]]
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[[Category: Ogato C]]
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[[Category: Palczewski, K.]]
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[[Category: Palczewski K]]
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[[Category: Wang, D.]]
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[[Category: Wang D]]
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[[Category: Xu, Z.]]
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[[Category: Xu Z]]
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[[Category: Young, N M.]]
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[[Category: Young NM]]
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[[Category: 3-galnac]]
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[[Category: Gal-beta-1]]
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[[Category: Lectin]]
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[[Category: Maclura pomifera agglutinin]]
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[[Category: Mpa]]
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[[Category: Mpa complex]]
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[[Category: Sugar binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 22 09:03:03 2010''
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Current revision

Crystal Structure Analysis of Maclura pomifera agglutinin complex with Gal-beta-1,3-GalNAc

PDB ID 3llz

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