2h2k

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[[Image:2h2k.jpg|left|200px]]<br /><applet load="2h2k" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2h2k, resolution 2.&Aring;" />
 
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'''Crystal Structure Analysis of Human S100A13'''<br />
 
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==Overview==
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==Crystal Structure Analysis of Human S100A13==
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The S100 protein family is the largest group of calcium-binding protein, families, which consists of at least 25 members. S100A13, which is widely, expressed in a variety of tissues, is a unique member of the S100 protein, family. Previous reports showed that S100A13 might be involved in the, stress-induced release of some signal peptide-less proteins (such as FGF-1, and IL-1alpha) and also associated with inflammatory functions. It was, also reported that S100A13 is a new angiogenesis marker. Here we report, the crystal structure of the Ca(2+)-bound form of S100A13 at 2.0 A, resolution. S100A13 is a homodimer with four EF-hand motifs in an, asymmetric unit, displaying a folding pattern similar to other S100, members. However, S100A13 has the unique structural feature with all, alpha-helices being amphiphilic, which was not found in other members of, S100s. We propose that this characteristic structure of S100A13 might be, related to its ability to mediate the release of FGF-1 and IL-1alpha.
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<StructureSection load='2h2k' size='340' side='right'caption='[[2h2k]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2h2k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H2K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h2k OCA], [https://pdbe.org/2h2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h2k RCSB], [https://www.ebi.ac.uk/pdbsum/2h2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h2k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/S10AD_HUMAN S10AD_HUMAN] Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine (By similarity).<ref>PMID:12746488</ref> <ref>PMID:20863990</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h2/2h2k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h2k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The S100 protein family is the largest group of calcium-binding protein families, which consists of at least 25 members. S100A13, which is widely expressed in a variety of tissues, is a unique member of the S100 protein family. Previous reports showed that S100A13 might be involved in the stress-induced release of some signal peptide-less proteins (such as FGF-1 and IL-1alpha) and also associated with inflammatory functions. It was also reported that S100A13 is a new angiogenesis marker. Here we report the crystal structure of the Ca(2+)-bound form of S100A13 at 2.0 A resolution. S100A13 is a homodimer with four EF-hand motifs in an asymmetric unit, displaying a folding pattern similar to other S100 members. However, S100A13 has the unique structural feature with all alpha-helices being amphiphilic, which was not found in other members of S100s. We propose that this characteristic structure of S100A13 might be related to its ability to mediate the release of FGF-1 and IL-1alpha.
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==About this Structure==
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Crystal structure study on human S100A13 at 2.0 A resolution.,Li M, Zhang PF, Pan XW, Chang WR Biochem Biophys Res Commun. 2007 May 11;356(3):616-21. Epub 2007 Mar 12. PMID:17374362<ref>PMID:17374362</ref>
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2H2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H2K OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure study on human S100A13 at 2.0 A resolution., Li M, Zhang PF, Pan XW, Chang WR, Biochem Biophys Res Commun. 2007 May 11;356(3):616-21. Epub 2007 Mar 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17374362 17374362]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 2h2k" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Chang, W.R.]]
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[[Category: Li, M.]]
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[[Category: Zhang, J.P.]]
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[[Category: Zhang, P.F.]]
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[[Category: CA]]
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[[Category: calcium binding protein]]
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[[Category: metal binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:12:26 2008''
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==See Also==
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*[[S100 proteins 3D structures|S100 proteins 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Chang WR]]
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[[Category: Li M]]
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[[Category: Zhang JP]]
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[[Category: Zhang PF]]

Current revision

Crystal Structure Analysis of Human S100A13

PDB ID 2h2k

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