2z6t

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(New page: 200px<br /><applet load="2z6t" size="350" color="white" frame="true" align="right" spinBox="true" caption="2z6t, resolution 1.20&Aring;" /> '''Crystal structure of...)
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[[Image:2z6t.jpg|left|200px]]<br /><applet load="2z6t" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2z6t, resolution 1.20&Aring;" />
 
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'''Crystal structure of the ferric peroxo myoglobin'''<br />
 
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==Overview==
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==Crystal structure of the ferric peroxo myoglobin==
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Crystals of the ferric peroxo (Fe3+-O-O-) species of myoglobin (Mb) have, been successfully generated by radiolytic reduction of the oxy Mb crystals, at 100 K by irradiation with 1.0 A synchrotron radiation. X-ray, diffraction using 0.6 A synchrotron radiation has permitted, for the first, time, the determination of the crystal structure of the ferric peroxo heme, species, an important intermediate in mono-oxygenase heme enzyme, catalysis. The Fe-O-O geometry is similar to that found in the ferrous oxy, Mb, the crystal structure of which has been determined also by the 0.6 A, radiation. The optical absorption spectra of the crystals affirm that oxy, and peroxo species have been maintained during the diffraction, measurements, indicating that adverse photoreduction by the incident beam, could be mostly avoided by use of the 0.6 A wavelength synchrotron, radiation for the diffraction measurements. The Fe-O-O geometries of the, oxy and peroxo Mb found in the crystal structures are supported by the, QM/MM calculations, which also show that the existence of a strong, hydrogen bonding interaction between NepsilonH of His64 and distal oxygen, atom in both the oxy and peroxo Mb. The ground state of the peroxo Mb is, found as a doublet state, while that of the oxy Mb is an open-shell, singlet state with two unpaired alpha and beta electrons mainly, distributed on the Fe and O2 moiety.
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<StructureSection load='2z6t' size='340' side='right'caption='[[2z6t]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2z6t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z6T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PER:PEROXIDE+ION'>PER</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z6t OCA], [https://pdbe.org/2z6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z6t RCSB], [https://www.ebi.ac.uk/pdbsum/2z6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z6t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYG_PHYMC MYG_PHYMC] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z6/2z6t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z6t ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2Z6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=PER:'>PER</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6T OCA].
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*[[Myoglobin 3D structures|Myoglobin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural Characterization of the Fleeting Ferric Peroxo Species in Myoglobin: Experiment and Theory., Unno M, Chen H, Kusama S, Shaik S, Ikeda-Saito M, J Am Chem Soc. 2007 Nov 7;129(44):13394-13395. Epub 2007 Oct 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17929929 17929929]
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[[Category: Large Structures]]
[[Category: Physeter catodon]]
[[Category: Physeter catodon]]
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[[Category: Single protein]]
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[[Category: Chen H]]
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[[Category: Chen, H.]]
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[[Category: Ikeda-Saito M]]
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[[Category: Ikeda-Saito, M.]]
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[[Category: Kusama S]]
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[[Category: Kusama, S.]]
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[[Category: Shaik S]]
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[[Category: Shaik, S.]]
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[[Category: Unno M]]
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[[Category: Unno, M.]]
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[[Category: HEM]]
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[[Category: PER]]
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[[Category: SO4]]
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[[Category: heme]]
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[[Category: iron]]
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[[Category: metal-binding]]
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[[Category: microspectrophotometer]]
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[[Category: muscle protein]]
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[[Category: oxygen binding]]
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[[Category: oxygen transport]]
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[[Category: peroxo]]
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[[Category: transport]]
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[[Category: x-ray-induced-photoreduction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:13:34 2008''
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Current revision

Crystal structure of the ferric peroxo myoglobin

PDB ID 2z6t

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