Basic Pancreatic Trypsin Inhibitor

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[[Image:1bpi.png|left|200px|thumb|Crystal Structure of BPTI [[1bpi]]]]
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<StructureSection load='3tgi' size='350' side='right' scene='43/430026/Cv/2' caption='Bovine BPTI (in salmon) complex with trypsin (magenta), sulfate and Ca+2 ion (green) (PDB code [[3tgi]])'>
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{{STRUCTURE_1bpi| PDB=1bpi | SIZE=300| SCENE=Basic_Pancreatic_Trypsin_Inhibitor/Cv/1 |right|CAPTION=BPTI [[1bpi]] }}
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[[Basic Pancreatic Trypsin Inhibitor]] (BPTI) is a small protein which inhibits trypsin and related proteases. The images at the left and at the right correspond to one representative BPTI, ''i.e.'' the crystal structure of bovine
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== Function ==
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BPTI ([[1bpi]]).
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{{TOC limit|limit=2}}
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[[Basic Pancreatic Trypsin Inhibitor]] (BPTI) is a small protein which inhibits trypsin and related proteases. <ref>PMID:15299722</ref> For other trypsin inhibitors see [[Trypsin inhibitor]].
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== 3D Structures of BPTI ==
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== Relevance ==
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=== BPTI ===
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BPTI is used as drug called Aprotinin used to reduce bleeding during surgery.
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[[2zvx]], [[2zjx]], [[1g6x]], [[1qlq]], [[1bti]], [[1fan]], [[1nag]], [[1bpt]], [[7pti]], [[8pti]] – bBPTI (mutant) - bovine<br />
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== Structural highlights ==
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[[3ci7]] - BPTI (mutant) – synthesized<br />
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[[2by5]], [[2by6]], [[2by7]], [[2by8]], [[2by9]], [[2bya]], [[1k6u]], [[1bhc]], [[1bpi]] - bBPTI<br />
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[[1uua]] – bBPTI truncated – NMR<br />
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[[1uub]] - bBPTI truncated (mutant) – NMR<br />
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[[1oa5]], [[1oa6]] – bBPTI – NMR<br />
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[[1ld5]], [[1ld6]], [[1jv8]], [[1jv9]] - bBPTI (mutant) – NMR<br />
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<scene name='43/430026/Cv/3'>Residue K15 of BPTI</scene> extends into the active site of trypsin and inhibits its proteolytic function. <ref>PMID:10210204</ref>
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== 3D Structures of BPTI ==
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[[BPTI 3D structures]]
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=== BPTI+protease ===
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</StructureSection>
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[[3fp6]] – rBPTI+anionic trypsin – rat<br />
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[[3tgi]] - bBPTI+anionic trypsin<br />
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[[1co7]], [[1f5r]], [[1f7z]], [[3tgk]] - rBPTI+anionic trypsin (mutant)<br />
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[[3tgj]] - bBPTI+anionic trypsin (mutant)<br />
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[[3fp7]], [[3fp8]], [[1fy8]] – rBPTI (mutant) +anionic trypsin<br />
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[[2r9p]] – bBPTI+hMesotrypsin (mutant)<br />
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[[2ra3]] - bBPTI+hTrypsin cationic (mutant)<br />
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[[2ijo]] – bBPTI+West Nile virus protease<br />
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[[1ykt]] – bBPTI+rTrypsin II (mutant)<br />
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[[1ylc]], [[1yld]] - bBPTI (mutant)+ rTrypsin II (mutant)<br />
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[[2ftl]], [[3btk]] - bBPTI+bTrypsin cationic <br />
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[[1brb]] - bBPTI+rTrypsin cationic<br />
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[[2ftm]], [[2fi3]], [[2fi5]], [[1ejm]] – bBPTI (mutant)+bTrypsin cationic<br />
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[[3bte]], [[3btq]], [[3btd]], [[3btf]], [[3btg]], [[3bth]], [[3btm]], [[3btt]], [[3btw]], [[1aal]], [[6pti]] - bBPTI (mutant)+bTrypsin<br />
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[[2fi4]] - bBPTI (mutant)+bTrypsin cationic (mutant)<br />
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[[1t7c]], [[1t8l]] - bBPTI (mutant)+bChymotrypsin <br />
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[[1cbw]], [[1mtn]] - bBPTI+bChymotrypsin A<br />
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[[1t8m]], [[1t8n]], [[1t8o]] - bBPTI +bChymotrypsin A (mutant)<br />
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[[1p2i]], [[1p2j]], [[1p2k]] - bBPTI (mutant)+bTrypsinogen<br />
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[[1p2m]], [[1p2n]], [[1p2o]], [[1p2q]] - bBPTI (mutant)+bChymotrypsinogen A<br />
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[[1eaw]] – bBPTI+hMatriptase<br />
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[[1d0d]] – bBPTI+anticoagulant protein – tick
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Bovine BPTI (in salmon) complex with trypsin (magenta), sulfate and Ca+2 ion (green) (PDB code 3tgi)

Drag the structure with the mouse to rotate

References

  1. ↑ Parkin S, Rupp B, Hope H. Structure of bovine pancreatic trypsin inhibitor at 125 K definition of carboxyl-terminal residues Gly57 and Ala58. Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):18-29. PMID:15299722 doi:http://dx.doi.org/10.1107/S0907444995008675
  2. ↑ Pasternak A, Ringe D, Hedstrom L. Comparison of anionic and cationic trypsinogens: the anionic activation domain is more flexible in solution and differs in its mode of BPTI binding in the crystal structure. Protein Sci. 1999 Jan;8(1):253-8. PMID:10210204

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Jaime Prilusky

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