8pti

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[[Image:8pti.png|left|200px]]
 
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==CRYSTAL STRUCTURE OF A Y35G MUTANT OF BOVINE PANCREATIC TRYPSIN INHIBITOR==
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The line below this paragraph, containing "STRUCTURE_8pti", creates the "Structure Box" on the page.
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<StructureSection load='8pti' size='340' side='right'caption='[[8pti]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[8pti]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PTI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PTI FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pti OCA], [https://pdbe.org/8pti PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pti RCSB], [https://www.ebi.ac.uk/pdbsum/8pti PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pti ProSAT]</span></td></tr>
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{{STRUCTURE_8pti| PDB=8pti | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/8pti_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=8pti ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of a Y35G mutant of bovine pancreatic trypsin inhibitor (BPTI) was solved by molecular replacement and was refined by both simulated annealing and restrained least-squares at 1.8 A resolution. The crystals belong to the space group P42212, with unit cell dimensions a = b = 46.75 A, c = 50.61 A. The final R-factor is 0.159 and the deviation from ideality for bond distances is 0.02 A. The structure of the mutant differs from that of the native protein, showing an overall root-mean-square (r.m.s.) difference of 1.86 A for main-chain atoms. However, the change is mostly localized in the two loops (respective r.m.s. values of 2.04 A and 3.93 A) and the C terminus (r.m.s. 6.79 A), while the core of the protein is well conserved (r.m.s. 0.45 A). The change in the loop regions can be clearly attributed to the mutation while the difference in the C terminus might be only due to a different crystal packing. Seventy water molecules were included in the model but only seven of them are shared with the native structure. Thermal parameters are showing a good correlation with those for the wild-type of BPTI.
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===CRYSTAL STRUCTURE OF A Y35G MUTANT OF BOVINE PANCREATIC TRYPSIN INHIBITOR===
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Crystal structure of a Y35G mutant of bovine pancreatic trypsin inhibitor.,Housset D, Kim KS, Fuchs J, Woodward C, Wlodawer A J Mol Biol. 1991 Aug 5;220(3):757-70. PMID:1714504<ref>PMID:1714504</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_1714504}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 8pti" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 1714504 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1714504}}
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==About this Structure==
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[[8pti]] is a 1 chain structure of [[Basic_Pancreatic_Trypsin_Inhibitor]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PTI OCA].
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==See Also==
==See Also==
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*[[Basic_Pancreatic_Trypsin_Inhibitor]]
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*[[BPTI 3D structures|BPTI 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:1714504</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Fuchs, J.]]
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[[Category: Large Structures]]
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[[Category: Housset, D.]]
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[[Category: Fuchs J]]
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[[Category: Kim, K S.]]
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[[Category: Housset D]]
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[[Category: Wlodawer, A.]]
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[[Category: Kim K-S]]
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[[Category: Woodward, C.]]
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[[Category: Wlodawer A]]
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[[Category: Woodward C]]

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CRYSTAL STRUCTURE OF A Y35G MUTANT OF BOVINE PANCREATIC TRYPSIN INHIBITOR

PDB ID 8pti

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