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2qj3
From Proteopedia
(Difference between revisions)
(New page: 200px<br /><applet load="2qj3" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qj3, resolution 3.00Å" /> '''Mycobacterium tuberc...) |
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| - | [[Image:2qj3.jpg|left|200px]]<br /><applet load="2qj3" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2qj3, resolution 3.00Å" /> | ||
| - | '''Mycobacterium tuberculosis FabD'''<br /> | ||
| - | == | + | ==Mycobacterium tuberculosis FabD== |
| - | Mycobacteria display a unique and unusual cell-wall architecture, central | + | <StructureSection load='2qj3' size='340' side='right'caption='[[2qj3]], [[Resolution|resolution]] 3.00Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2qj3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QJ3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fabD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/[Acyl-carrier-protein]_S-malonyltransferase [Acyl-carrier-protein] S-malonyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.39 2.3.1.39] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qj3 OCA], [https://pdbe.org/2qj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qj3 RCSB], [https://www.ebi.ac.uk/pdbsum/2qj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qj3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qj/2qj3_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qj3 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mycobacteria display a unique and unusual cell-wall architecture, central to which is the membrane-proximal mycolyl-arabinogalactan-peptidoglycan core (mAGP). The biosynthesis of mycolic acids, which form the outermost layer of the mAGP core, involves malonyl-CoA:acyl carrier protein transacylase (MCAT). This essential enzyme catalyses the transfer of malonyl from coenzyme A to acyl carrier protein AcpM, thus feeding these two-carbon units into the chain-elongation cycle of the type II fatty-acid synthase. The crystal structure of M. tuberculosis mtFabD, the mycobacterial MCAT, has been determined to 3.0 A resolution by multi-wavelength anomalous dispersion. Phasing was facilitated by Ni2+ ions bound to the 20-residue N-terminal affinity tag, which packed between the two independent copies of mtFabD. | ||
| - | + | Structure of Mycobacterium tuberculosis mtFabD, a malonyl-CoA:acyl carrier protein transacylase (MCAT).,Ghadbane H, Brown AK, Kremer L, Besra GS, Futterer K Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Oct 1;63(Pt, 10):831-5. Epub 2007 Sep 19. PMID:17909282<ref>PMID:17909282</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 2qj3" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | + | <references/> | |
| - | [[Category: Besra, G | + | __TOC__ |
| - | [[Category: Brown, A | + | </StructureSection> |
| - | [[Category: Futterer, K | + | [[Category: Large Structures]] |
| - | [[Category: Ghadbane, H | + | [[Category: Myctu]] |
| - | [[Category: Kremer, L | + | [[Category: Besra, G S]] |
| - | [[Category: | + | [[Category: Brown, A K]] |
| - | + | [[Category: Futterer, K]] | |
| - | [[Category: | + | [[Category: Ghadbane, H]] |
| - | [[Category: | + | [[Category: Kremer, L]] |
| - | [[Category: | + | [[Category: Fatty acid synthase]] |
| - | + | [[Category: Malonyl-coa]] | |
| - | + | [[Category: Mycolic acid]] | |
| + | [[Category: Transferase]] | ||
Current revision
Mycobacterium tuberculosis FabD
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