2w2e

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[[Image:2w2e.png|left|200px]]
 
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==1.15 Angstrom crystal structure of P.pastoris aquaporin, Aqy1, in a closed conformation at pH 3.5==
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The line below this paragraph, containing "STRUCTURE_2w2e", creates the "Structure Box" on the page.
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<StructureSection load='2w2e' size='340' side='right'caption='[[2w2e]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w2e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_pastoris Komagataella pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W2E FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_2w2e| PDB=2w2e | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w2e OCA], [https://pdbe.org/2w2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w2e RCSB], [https://www.ebi.ac.uk/pdbsum/2w2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w2e ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/F2QVG4_KOMPC F2QVG4_KOMPC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w2/2w2e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w2e ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Aquaporins are transmembrane proteins that facilitate the flow of water through cellular membranes. An unusual characteristic of yeast aquaporins is that they frequently contain an extended N terminus of unknown function. Here we present the X-ray structure of the yeast aquaporin Aqy1 from Pichia pastoris at 1.15 A resolution. Our crystal structure reveals that the water channel is closed by the N terminus, which arranges as a tightly wound helical bundle, with Tyr31 forming H-bond interactions to a water molecule within the pore and thereby occluding the channel entrance. Nevertheless, functional assays show that Aqy1 has appreciable water transport activity that aids survival during rapid freezing of P. pastoris. These findings establish that Aqy1 is a gated water channel. Mutational studies in combination with molecular dynamics simulations imply that gating may be regulated by a combination of phosphorylation and mechanosensitivity.
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===1.15 ANGSTROM CRYSTAL STRUCTURE OF P.PASTORIS AQUAPORIN, AQY1, IN A CLOSED CONFORMATION AT PH 3.5===
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Crystal structure of a yeast aquaporin at 1.15 angstrom reveals a novel gating mechanism.,Fischer G, Kosinska-Eriksson U, Aponte-Santamaria C, Palmgren M, Geijer C, Hedfalk K, Hohmann S, de Groot BL, Neutze R, Lindkvist-Petersson K PLoS Biol. 2009 Jun;7(6):e1000130. Epub 2009 Jun 16. PMID:19529756<ref>PMID:19529756</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[2w2e]] is a 1 chain structure of [[Aquaporin]] with sequence from [http://en.wikipedia.org/wiki/Pichia_pastoris Pichia pastoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W2E OCA].
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<div class="pdbe-citations 2w2e" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
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*[[Aquaporin]]
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*[[Aquaporin 3D structures|Aquaporin 3D structures]]
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[[Category: Pichia pastoris]]
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== References ==
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[[Category: Aponte-Santamaria, C.]]
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<references/>
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[[Category: Fischer, G.]]
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__TOC__
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[[Category: Geijer, C.]]
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</StructureSection>
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[[Category: Groot, B L.De.]]
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[[Category: Komagataella pastoris]]
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[[Category: Hedfalk, K.]]
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[[Category: Large Structures]]
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[[Category: Hohmann, S.]]
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[[Category: Aponte-Santamaria C]]
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[[Category: Kosinska-Eriksson, U.]]
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[[Category: Fischer G]]
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[[Category: Lindkvist-Petersson, K.]]
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[[Category: Geijer C]]
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[[Category: Neutze, R.]]
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[[Category: Hedfalk K]]
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[[Category: Palmgren, M.]]
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[[Category: Hohmann S]]
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[[Category: Aquaporin]]
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[[Category: Kosinska-Eriksson U]]
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[[Category: Gating]]
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[[Category: Lindkvist-Petersson K]]
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[[Category: Membrane protein]]
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[[Category: Neutze R]]
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[[Category: Yeast]]
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[[Category: Palmgren M]]
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[[Category: De Groot BL]]

Current revision

1.15 Angstrom crystal structure of P.pastoris aquaporin, Aqy1, in a closed conformation at pH 3.5

PDB ID 2w2e

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