2qrt

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(New page: 200px<br /><applet load="2qrt" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qrt, resolution 1.80&Aring;" /> '''Crystal Structure of...)
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[[Image:2qrt.jpg|left|200px]]<br /><applet load="2qrt" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qrt, resolution 1.80&Aring;" />
 
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'''Crystal Structure of a disulfide trapped single chain trimer composed of the MHC I heavy chain H-2Kb Y84C, beta-2microglobulin, and ovalbumin-derived peptide.'''<br />
 
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==Overview==
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==Crystal Structure of a disulfide trapped single chain trimer composed of the MHC I heavy chain H-2Kb Y84C, beta-2microglobulin, and ovalbumin-derived peptide.==
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MHC class I peptide complexes (pMHC) are routinely used to enumerate T, cell populations and are currently being evaluated as vaccines to tumors, and specific pathogens. Herein, we describe the structures of three, generations of single-chain pMHC progressively designed for the optimal, presentation of covalently associated epitopes. Our ultimate design, employs a versatile disulfide trap between an invariant MHC residue and a, short C-terminal peptide extension. This general strategy is nondisruptive, of native pMHC conformation and T cell receptor engagement. Indeed, cell-surface-expressed MHC complexes with disulfide-trapped epitopes are, refractory to peptide exchange, suggesting they will make safe and, effective vaccines. Furthermore, we find that disulfide-trap stabilized, recombinant pMHC reagents reliably detect polyclonal CD8 T cell, populations as proficiently as conventional reagents and are thus well, suited to monitor or modulate immune responses during pathogenesis.
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<StructureSection load='2qrt' size='340' side='right'caption='[[2qrt]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2qrt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QRT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qrt OCA], [https://pdbe.org/2qrt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qrt RCSB], [https://www.ebi.ac.uk/pdbsum/2qrt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qrt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HA1B_MOUSE HA1B_MOUSE] Involved in the presentation of foreign antigens to the immune system.[https://www.uniprot.org/uniprot/B2MG_MOUSE B2MG_MOUSE] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/2qrt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qrt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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MHC class I peptide complexes (pMHC) are routinely used to enumerate T cell populations and are currently being evaluated as vaccines to tumors and specific pathogens. Herein, we describe the structures of three generations of single-chain pMHC progressively designed for the optimal presentation of covalently associated epitopes. Our ultimate design employs a versatile disulfide trap between an invariant MHC residue and a short C-terminal peptide extension. This general strategy is nondisruptive of native pMHC conformation and T cell receptor engagement. Indeed, cell-surface-expressed MHC complexes with disulfide-trapped epitopes are refractory to peptide exchange, suggesting they will make safe and effective vaccines. Furthermore, we find that disulfide-trap stabilized, recombinant pMHC reagents reliably detect polyclonal CD8 T cell populations as proficiently as conventional reagents and are thus well suited to monitor or modulate immune responses during pathogenesis.
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==About this Structure==
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Structural engineering of pMHC reagents for T cell vaccines and diagnostics.,Mitaksov V, Truscott SM, Lybarger L, Connolly JM, Hansen TH, Fremont DH Chem Biol. 2007 Aug;14(8):909-22. PMID:17719490<ref>PMID:17719490</ref>
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2QRT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRT OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural engineering of pMHC reagents for T cell vaccines and diagnostics., Mitaksov V, Truscott SM, Lybarger L, Connolly JM, Hansen TH, Fremont DH, Chem Biol. 2007 Aug;14(8):909-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17719490 17719490]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 2qrt" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Fremont, D.H.]]
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[[Category: Mitaksov, V.E.]]
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[[Category: glycoprotein]]
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[[Category: immune response]]
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[[Category: immune system]]
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[[Category: membrane]]
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[[Category: mhc i]]
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[[Category: mhc-i]]
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[[Category: ova]]
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[[Category: single chain mhc-i]]
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[[Category: transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:23:50 2008''
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==See Also==
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*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
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*[[MHC 3D structures|MHC 3D structures]]
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*[[MHC I 3D structures|MHC I 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Fremont DH]]
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[[Category: Mitaksov VE]]

Current revision

Crystal Structure of a disulfide trapped single chain trimer composed of the MHC I heavy chain H-2Kb Y84C, beta-2microglobulin, and ovalbumin-derived peptide.

PDB ID 2qrt

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OCA

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