1it5

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[[Image:1it5.png|left|200px]]
 
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==Solution structure of apo-type PLA2 from Streptomyces violaceruber A-2688.==
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The line below this paragraph, containing "STRUCTURE_1it5", creates the "Structure Box" on the page.
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<StructureSection load='1it5' size='340' side='right'caption='[[1it5]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1it5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_violaceoruber Streptomyces violaceoruber]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IT5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 1 model</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1it5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1it5 OCA], [https://pdbe.org/1it5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1it5 RCSB], [https://www.ebi.ac.uk/pdbsum/1it5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1it5 ProSAT]</span></td></tr>
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{{STRUCTURE_1it5| PDB=1it5 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6UV28_STRVN Q6UV28_STRVN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/it/1it5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1it5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Until now, phospholipase A(2) (PLA(2); EC 3.1.14) has been found only from eukaryotic sources. In the present study, we found a secreted PLA(2), which is produced by a soil bacterium, Streptomyces violaceoruber A-2688, demonstrating that the enzyme is the first phospholipase A(2) identified in prokaryote. After characterization of the novel PLA(2), a gene encoding the enzyme was cloned, sequenced, and overexpressed using a Streptomyces host-vector system. The amino acid sequence showed that the prokaryotic PLA(2) has only four cysteines and less homology to the eukaryotic ones, which have 12-16 cysteines. The solution structures of the prokaryotic PLA(2), bound and unbound with calcium(II) ion, were determined by using the NMR technique and structure calculation. The overall structure of the S. violaceoruber PLA(2), which is composed of only five alpha-helices, is completely different from those of eukaryotic PLA(2)s, which consist of beta-sheets and alpha-helices. The structure of the calcium-binding domain is obviously distinct from that without the ion; the ligands for the calcium(II) ion are the two carboxylates of Asp(43) (monodentate) and Asp(65) (bidentate), the carbonyl oxygen of Leu(44), and three water molecules. A calcium-binding experiment showed that the calcium dissociation constant ( approximately 5 mm) for the prokaryotic PLA(2) is much larger than those of eukaryotic ones.
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===Solution structure of apo-type PLA2 from Streptomyces violaceruber A-2688.===
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A novel prokaryotic phospholipase A2. Characterization, gene cloning, and solution structure.,Sugiyama M, Ohtani K, Izuhara M, Koike T, Suzuki K, Imamura S, Misaki H J Biol Chem. 2002 May 31;277(22):20051-8. Epub 2002 Mar 15. PMID:11897786<ref>PMID:11897786</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_11897786}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1it5" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 11897786 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11897786}}
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==About this Structure==
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[[1it5]] is a 1 chain structure of [[Phospholipase A2]] with sequence from [http://en.wikipedia.org/wiki/Streptomyces_violaceoruber Streptomyces violaceoruber]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IT5 OCA].
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==See Also==
==See Also==
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*[[Phospholipase A2]]
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*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:11897786</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces violaceoruber]]
[[Category: Streptomyces violaceoruber]]
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[[Category: Izuhara, M.]]
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[[Category: Izuhara M]]
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[[Category: Koike, T.]]
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[[Category: Koike T]]
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[[Category: Ohtani, K.]]
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[[Category: Ohtani K]]
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[[Category: Sugiyama, M.]]
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[[Category: Sugiyama M]]
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[[Category: Hydrolase]]
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[[Category: Prokaryotic pla2]]
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Current revision

Solution structure of apo-type PLA2 from Streptomyces violaceruber A-2688.

PDB ID 1it5

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