This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3d3c

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:39, 30 August 2023) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3d3c.png|left|200px]]
 
-
<!--
+
==Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.==
-
The line below this paragraph, containing "STRUCTURE_3d3c", creates the "Structure Box" on the page.
+
<StructureSection load='3d3c' size='340' side='right'caption='[[3d3c]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3d3c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3D3C FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3d3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3d3c OCA], [https://pdbe.org/3d3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3d3c RCSB], [https://www.ebi.ac.uk/pdbsum/3d3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3d3c ProSAT]</span></td></tr>
-
{{STRUCTURE_3d3c| PDB=3d3c | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/NUSB_ECOLI NUSB_ECOLI] One of the proteins essential for the formation of the RNA polymerase antitermination complex in the presence of lambda phage N protein. However, it is involved in the transcription termination process at certain sites during normal bacterial growth. Binds to the BoxA RNA motif.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/3d3c_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3d3c ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Protein S10 is a component of the 30S ribosomal subunit and participates together with NusB protein in processive transcription antitermination. The molecular mechanisms by which S10 can act as a translation or a transcription factor are not understood. We used complementation assays and recombineering to delineate regions of S10 dispensable for antitermination, and determined the crystal structure of a transcriptionally active NusB-S10 complex. In this complex, S10 adopts the same fold as in the 30S subunit and is blocked from simultaneous association with the ribosome. Mass spectrometric mapping of UV-induced crosslinks revealed that the NusB-S10 complex presents an intermolecular, composite, and contiguous binding surface for RNAs containing BoxA antitermination signals. Furthermore, S10 overproduction complemented a nusB null phenotype. These data demonstrate that S10 and NusB together form a BoxA-binding module, that NusB facilitates entry of S10 into the transcription machinery, and that S10 represents a central hub in processive antitermination.
-
===Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.===
+
Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.,Luo X, Hsiao HH, Bubunenko M, Weber G, Court DL, Gottesman ME, Urlaub H, Wahl MC Mol Cell. 2008 Dec 26;32(6):791-802. PMID:19111659<ref>PMID:19111659</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_19111659}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 3d3c" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 19111659 is the PubMed ID number.
+
-
-->
+
-
{{ABSTRACT_PUBMED_19111659}}
+
-
 
+
-
==About this Structure==
+
-
[[3d3c]] is a 6 chain structure of [[Ribosomal protein S10]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D3C OCA].
+
==See Also==
==See Also==
-
*[[Ribosomal protein S10]]
+
*[[Ribosomal protein S10|Ribosomal protein S10]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:19111659</ref><references group="xtra"/>
+
__TOC__
-
[[Category: Escherichia coli k12]]
+
</StructureSection>
-
[[Category: Luo, X.]]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Wahl, M C.]]
+
[[Category: Large Structures]]
-
[[Category: Lambda n antitermination]]
+
[[Category: Luo X]]
-
[[Category: Nusb]]
+
[[Category: Wahl MC]]
-
[[Category: Nuse]]
+
-
[[Category: Nut]]
+
-
[[Category: Phage lambda]]
+
-
[[Category: Ribonucleoprotein]]
+
-
[[Category: Ribosomal protein]]
+
-
[[Category: Rna-binding]]
+
-
[[Category: Rrn antitermination]]
+
-
[[Category: S10]]
+
-
[[Category: Transcription]]
+
-
[[Category: Transcription regulation]]
+
-
[[Category: Transcription termination]]
+

Current revision

Structural and functional analysis of the E. coli NusB-S10 transcription antitermination complex.

PDB ID 3d3c

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3d3c"
Personal tools