2k9c

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[[Image:2k9c.png|left|200px]]
 
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==Paramagnetic shifts in solid-state NMR of Proteins to elicit structural information==
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The line below this paragraph, containing "STRUCTURE_2k9c", creates the "Structure Box" on the page.
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<StructureSection load='2k9c' size='340' side='right'caption='[[2k9c]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2k9c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K9C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K9C FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
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{{STRUCTURE_2k9c| PDB=2k9c | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k9c OCA], [https://pdbe.org/2k9c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k9c RCSB], [https://www.ebi.ac.uk/pdbsum/2k9c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k9c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MMP12_HUMAN MMP12_HUMAN] May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The recent observation of pseudocontact shifts (pcs) in (13)C high-resolution solid-state NMR of paramagnetic proteins opens the way to their application as structural restraints. Here, by investigating a microcrystalline sample of cobalt(II)-substituted matrix metalloproteinase 12 [CoMMP-12 (159 AA, 17.5 kDa)], it is shown that a combined strategy of protein labeling and dilution of the paramagnetic species (i.e., (13)C-,(15)N-labeled CoMMP-12 diluted in unlabeled ZnMMP-12, and (13)C-,(15)N-labeled ZnMMP-12 diluted in unlabeled CoMMP-12) allows one to easily separate the pcs contributions originated from the protein internal metal (intramolecular pcs) from those due to the metals in neighboring proteins in the crystal lattice (intermolecular pcs) and that both can be used for structural purposes. It is demonstrated that intramolecular pcs are significant structural restraints helpful in increasing both precision and accuracy of the structure, which is a need in solid-state structural biology nowadays. Furthermore, intermolecular pcs provide unique information on positions and orientations of neighboring protein molecules in the solid phase.
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===Paramagnetic shifts in solid-state NMR of Proteins to elicit structural information===
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Paramagnetic shifts in solid-state NMR of proteins to elicit structural information.,Balayssac S, Bertini I, Bhaumik A, Lelli M, Luchinat C Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17284-9. Epub 2008 Nov 6. PMID:18988744<ref>PMID:18988744</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2k9c" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18988744 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18988744}}
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==About this Structure==
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[[2k9c]] is a 1 chain structure of [[Elastase]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K9C OCA].
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==See Also==
==See Also==
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*[[Elastase]]
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*[[Matrix metalloproteinase 3D structures|Matrix metalloproteinase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:18988744</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Macrophage elastase]]
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[[Category: Large Structures]]
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[[Category: Balayssac, S.]]
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[[Category: Balayssac S]]
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[[Category: Bertini, I.]]
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[[Category: Bertini I]]
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[[Category: Bhaumik, A.]]
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[[Category: Bhaumik A]]
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[[Category: Lelli, M.]]
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[[Category: Lelli M]]
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[[Category: Luchinat, C.]]
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[[Category: Luchinat C]]
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[[Category: Calcium]]
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[[Category: Extracellular matrix]]
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[[Category: Glycoprotein]]
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[[Category: Hydrolase]]
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[[Category: Matrix metalloproteinase]]
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[[Category: Metal-binding]]
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[[Category: Metalloprotease]]
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[[Category: Paramagnetic nmr]]
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[[Category: Polymorphism]]
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[[Category: Protease]]
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[[Category: Pseudocontact shift]]
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[[Category: Secreted]]
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[[Category: Solid-state nmr]]
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[[Category: Zinc]]
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[[Category: Zymogen]]
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Current revision

Paramagnetic shifts in solid-state NMR of Proteins to elicit structural information

PDB ID 2k9c

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