2nrm

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[[Image:2nrm.png|left|200px]]
 
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==S-nitrosylated blackfin tuna myoglobin==
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The line below this paragraph, containing "STRUCTURE_2nrm", creates the "Structure Box" on the page.
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<StructureSection load='2nrm' size='340' side='right'caption='[[2nrm]], [[Resolution|resolution]] 1.09&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2nrm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thunnus_atlanticus Thunnus atlanticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NRM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.09&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SNC:S-NITROSO-CYSTEINE'>SNC</scene></td></tr>
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{{STRUCTURE_2nrm| PDB=2nrm | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nrm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nrm OCA], [https://pdbe.org/2nrm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nrm RCSB], [https://www.ebi.ac.uk/pdbsum/2nrm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nrm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYG_THUTH MYG_THUTH] Monomeric heme protein which primary function is to store oxygen and facilitate its diffusion within muscle tissues. Reversibly binds oxygen through a pentacoordinated heme iron and enables its timely and efficient release as needed during periods of heightened demand. Depending on the oxidative conditions of tissues and cells, and in addition to its ability to bind oxygen, it also has a nitrite reductase activity whereby it regulates the production of bioactive nitric oxide. Under stress conditions, like hypoxia and anoxia, it also protects cells against reactive oxygen species thanks to its pseudoperoxidase activity.[UniProtKB:P02144]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nr/2nrm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nrm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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S-nitrosylation is a post-translational protein modification that can alter the function of a variety of proteins. Despite the growing wealth of information that this modification may have important functional consequences, little is known about the structure of the moiety or its effect on protein tertiary structure. Here we report high-resolution x-ray crystal structures of S-nitrosylated and unmodified blackfin tuna myoglobin, which demonstrate that in vitro S-nitrosylation of this protein at the surface-exposed Cys-10 directly causes a reversible conformational change by "wedging" apart a helix and loop. Furthermore, we have demonstrated in solution and in a single crystal that reduction of the S-nitrosylated myoglobin with dithionite results in NO cleavage from the sulfur of Cys-10 and rebinding to the reduced heme iron, showing the reversibility of both the modification and the conformational changes. Finally, we report the 0.95-A structure of ferrous nitrosyl myoglobin, which provides an accurate structural view of the NO coordination geometry in the context of a globin heme pocket.
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===S-nitrosylated blackfin tuna myoglobin===
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S-nitrosylation-induced conformational change in blackfin tuna myoglobin.,Schreiter ER, Rodriguez MM, Weichsel A, Montfort WR, Bonaventura J J Biol Chem. 2007 Jul 6;282(27):19773-80. Epub 2007 May 8. PMID:17488722<ref>PMID:17488722</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17488722}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2nrm" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17488722 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17488722}}
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==About this Structure==
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[[2nrm]] is a 1 chain structure of [[Myoglobin]] with sequence from [http://en.wikipedia.org/wiki/Thunnus_atlanticus Thunnus atlanticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NRM OCA].
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==See Also==
==See Also==
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*[[Myoglobin]]
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*[[Myoglobin 3D structures|Myoglobin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:17488722</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thunnus atlanticus]]
[[Category: Thunnus atlanticus]]
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[[Category: Bonaventura, J.]]
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[[Category: Bonaventura J]]
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[[Category: Montfort, W R.]]
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[[Category: Montfort WR]]
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[[Category: Schreiter, E R.]]
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[[Category: Rodr guez MM]]
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[[Category: Weichsel, A.]]
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[[Category: Schreiter ER]]
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[[Category: Guez, M M.Rodr.]]
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[[Category: Weichsel A]]
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[[Category: Globin]]
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[[Category: Myoglobin]]
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[[Category: S-nitrosylation]]
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[[Category: Transport protein]]
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Current revision

S-nitrosylated blackfin tuna myoglobin

PDB ID 2nrm

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