1nji

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[[Image:1nji.png|left|200px]]
 
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==Structure of chloramphenicol bound to the 50S ribosomal subunit==
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The line below this paragraph, containing "STRUCTURE_1nji", creates the "Structure Box" on the page.
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<StructureSection load='1nji' size='340' side='right'caption='[[1nji]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1nji]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. The January 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''70S Ribosomes'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_1 10.2210/rcsb_pdb/mom_2010_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NJI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CLM:CHLORAMPHENICOL'>CLM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_1nji| PDB=1nji | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nji OCA], [https://pdbe.org/1nji PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nji RCSB], [https://www.ebi.ac.uk/pdbsum/1nji PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nji ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL2_HALMA RL2_HALMA] One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome (By similarity).[HAMAP-Rule:MF_01320_A]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nj/1nji_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nji ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Structures of anisomycin, chloramphenicol, sparsomycin, blasticidin S, and virginiamycin M bound to the large ribosomal subunit of Haloarcula marismortui have been determined at 3.0A resolution. Most of these antibiotics bind to sites that overlap those of either peptidyl-tRNA or aminoacyl-tRNA, consistent with their functioning as competitive inhibitors of peptide bond formation. Two hydrophobic crevices, one at the peptidyl transferase center and the other at the entrance to the peptide exit tunnel play roles in binding these antibiotics. Midway between these crevices, nucleotide A2103 of H.marismortui (2062 Escherichia coli) varies in its conformation and thereby contacts antibiotics bound at either crevice. The aromatic ring of anisomycin binds to the active-site hydrophobic crevice, as does the aromatic ring of puromycin, while the aromatic ring of chloramphenicol binds to the exit tunnel hydrophobic crevice. Sparsomycin contacts primarily a P-site bound substrate, but also extends into the active-site hydrophobic crevice. Virginiamycin M occupies portions of both the A and P-site, and induces a conformational change in the ribosome. Blasticidin S base-pairs with the P-loop and thereby mimics C74 and C75 of a P-site bound tRNA.
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===Structure of chloramphenicol bound to the 50S ribosomal subunit===
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Structures of five antibiotics bound at the peptidyl transferase center of the large ribosomal subunit.,Hansen JL, Moore PB, Steitz TA J Mol Biol. 2003 Jul 25;330(5):1061-75. PMID:12860128<ref>PMID:12860128</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12860128}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1nji" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12860128 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12860128}}
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==About this Structure==
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[[1nji]] is a 30 chain structure of [[Ribosomal protein L10]], [[Ribosomal protein L2]], [[Ribosomal protein L3]], [[Ribosomal protein L5]], [[Ribosomal protein L6]], [[Ribosomal protein L7]] and [[Ribosome]] with sequence from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. The January 2010 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''70S Ribosomes'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2010_1 10.2210/rcsb_pdb/mom_2010_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJI OCA].
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==See Also==
==See Also==
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*[[Ribosomal protein L10]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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*[[Ribosomal protein L2]]
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== References ==
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*[[Ribosomal protein L3]]
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<references/>
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*[[Ribosomal protein L5]]
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__TOC__
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*[[Ribosomal protein L6]]
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</StructureSection>
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*[[Ribosomal protein L7]]
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*[[Ribosome]]
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==Reference==
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<ref group="xtra">PMID:12860128</ref><references group="xtra"/>
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[[Category: 70S Ribosomes]]
[[Category: 70S Ribosomes]]
[[Category: Haloarcula marismortui]]
[[Category: Haloarcula marismortui]]
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[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Hansen, J L.]]
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[[Category: Hansen JL]]
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[[Category: Moore, P B.]]
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[[Category: Moore PB]]
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[[Category: Steitz, T A.]]
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[[Category: Steitz TA]]
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[[Category: 50]]
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[[Category: Antibiotic]]
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[[Category: Chloramphenicol]]
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[[Category: Lsu]]
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[[Category: Ribosome]]
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Current revision

Structure of chloramphenicol bound to the 50S ribosomal subunit

PDB ID 1nji

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