3i3h

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[[Image:3i3h.png|left|200px]]
 
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==Crystal structure of Bothropstoxin-I crystallized at 291K==
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The line below this paragraph, containing "STRUCTURE_3i3h", creates the "Structure Box" on the page.
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<StructureSection load='3i3h' size='340' side='right'caption='[[3i3h]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3i3h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I3H FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.17&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i3h OCA], [https://pdbe.org/3i3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i3h RCSB], [https://www.ebi.ac.uk/pdbsum/3i3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i3h ProSAT]</span></td></tr>
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{{STRUCTURE_3i3h| PDB=3i3h | SCENE= }}
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</table>
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== Function ==
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===Crystal structure of Bothropstoxin-I crystallized at 291K===
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[https://www.uniprot.org/uniprot/PA2H1_BOTJR PA2H1_BOTJR] Snake venom phospholipase A2 homolog that lacks enzymatic activity. Shows local myotoxic activity (PubMed:11018293, PubMed:12079495, PubMed:31906173). Induces inflammation, since it induces edema and leukocytes infiltration (PubMed:11018293, PubMed:31906173). In addition, it induces NLRP3 NLRP3, ASC (PYCARD), caspase-1 (CASP1), and IL-1beta (IL1B) gene expression in the gastrocnemius muscle, showing that it is able to activate NLRP3 inflammasome (PubMed:31906173). It also damages artificial and myoblast membranes by a calcium-independent mechanism, has bactericidal activity, and induces neuromuscular blockade (PubMed:27531710). A model of myotoxic mechanism has been proposed: an apo Lys49-PLA2 is activated by the entrance of a hydrophobic molecule (e.g. fatty acid) at the hydrophobic channel of the protein leading to a reorientation of a monomer (By similarity) (PubMed:27531710). This reorientation causes a transition between 'inactive' to 'active' states, causing alignment of C-terminal and membrane-docking sites (MDoS) side-by-side and putting the membrane-disruption sites (MDiS) in the same plane, exposed to solvent and in a symmetric position for both monomers (By similarity) (PubMed:27531710). The MDoS region stabilizes the toxin on membrane by the interaction of charged residues with phospholipid head groups (By similarity) (PubMed:27531710). Subsequently, the MDiS region destabilizes the membrane with penetration of hydrophobic residues (By similarity) (PubMed:27531710). This insertion causes a disorganization of the membrane, allowing an uncontrolled influx of ions (i.e. calcium and sodium), and eventually triggering irreversible intracellular alterations and cell death (By similarity) (PubMed:27531710).[UniProtKB:I6L8L6]<ref>PMID:11018293</ref> <ref>PMID:11829743</ref> <ref>PMID:12079495</ref> <ref>PMID:17157889</ref> <ref>PMID:17346668</ref> <ref>PMID:18160090</ref> <ref>PMID:27531710</ref> <ref>PMID:3176051</ref> <ref>PMID:31906173</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==About this Structure==
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Check<jmol>
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[[3i3h]] is a 2 chain structure of [[Phospholipase A2]] with sequence from [http://en.wikipedia.org/wiki/Bothrops_jararacussu Bothrops jararacussu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I3H OCA].
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i3/3i3h_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i3h ConSurf].
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<div style="clear:both"></div>
==See Also==
==See Also==
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*[[Phospholipase A2]]
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*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
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*[[Phospholipase A2 homolog|Phospholipase A2 homolog]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bothrops jararacussu]]
[[Category: Bothrops jararacussu]]
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[[Category: Fontes, M R.M.]]
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[[Category: Large Structures]]
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[[Category: Marchi-Salvador, D P.]]
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[[Category: Fontes MRM]]
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[[Category: Salvador, G H.M.]]
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[[Category: Marchi-Salvador DP]]
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[[Category: Soares, A M.]]
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[[Category: Salvador GHM]]
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[[Category: Antimicrobial]]
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[[Category: Soares AM]]
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[[Category: Bothropstoxin-i]]
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[[Category: Bthtx-i_18c]]
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[[Category: Disulfide bond]]
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[[Category: Homologue phospholipase a2]]
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[[Category: Lys49-pla2 from bothrops jararacussu]]
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[[Category: Myotoxin]]
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[[Category: Secreted]]
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[[Category: Snake venom]]
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[[Category: Toxin]]
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Current revision

Crystal structure of Bothropstoxin-I crystallized at 291K

PDB ID 3i3h

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