2wg2

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[[Image:2wg2.png|left|200px]]
 
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==NON-AGED CONJUGATE OF TORPEDO CALIFORNICA ACETYLCHOLINESTERASE WITH SOMAN (ALTERNATIVE REFINEMENT)==
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The line below this paragraph, containing "STRUCTURE_2wg2", creates the "Structure Box" on the page.
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<StructureSection load='2wg2' size='340' side='right'caption='[[2wg2]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wg2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetronarce_californica Tetronarce californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WG2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GD8:(1R)-1,2,2-TRIMETHYLPROPYL+(S)-METHYLPHOSPHINATE'>GD8</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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{{STRUCTURE_2wg2| PDB=2wg2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wg2 OCA], [https://pdbe.org/2wg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wg2 RCSB], [https://www.ebi.ac.uk/pdbsum/2wg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wg2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACES_TETCF ACES_TETCF] Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/2wg2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wg2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Organophosphate compounds (OP) are potent inhibitors of acetylcholinesterases (AChEs) and can cause lethal poisoning in humans. Inhibition of AChEs by the OP soman involves phosphonylation of the catalytic serine, and subsequent dealkylation produces a form known as the "aged" enzyme. The nonaged form can be reactivated to a certain extent by nucleophiles, such as pralidoxime (2-PAM), whereas aged forms of OP-inhibited AChEs are totally resistant to reactivation. Here, we solved the X-ray crystal structures of AChE from Torpedo californica (TcAChE) conjugated with soman before and after aging. The absolute configuration of the soman stereoisomer adduct in the nonaged conjugate is P(S)C(R). A structural reorientation of the catalytic His440 side chain was observed during the aging process. Furthermore, the crystal structure of the ternary complex of the aged conjugate with 2-PAM revealed that the orientation of the oxime function does not permit nucleophilic attack on the phosphorus atom, thus providing a plausible explanation for its failure to reactivate the aged soman/AChE conjugate. Together, these three crystal structures provide an experimental basis for the design of new reactivators.
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===NON-AGED CONJUGATE OF TORPEDO CALIFORNICA ACETYLCHOLINESTERASE WITH SOMAN (ALTERNATIVE REFINEMENT)===
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Crystallographic Snapshots of Nonaged and Aged Conjugates of Soman with Acetylcholinesterase, and of a Ternary Complex of the Aged Conjugate with Pralidoxime (dagger).,Sanson B, Nachon F, Colletier JP, Froment MT, Toker L, Greenblatt HM, Sussman JL, Ashani Y, Masson P, Silman I, Weik M J Med Chem. 2009 Jul 30. PMID:19642642<ref>PMID:19642642</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_19642642}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2wg2" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19642642 is the PubMed ID number.
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{{ABSTRACT_PUBMED_19642642}}
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==About this Structure==
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[[2wg2]] is a 1 chain structure of [[Acetylcholinesterase]] with sequence from [http://en.wikipedia.org/wiki/Torpedo_californica Torpedo californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WG2 OCA].
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==See Also==
==See Also==
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*[[Acetylcholinesterase]]
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*[[Acetylcholinesterase 3D structures|Acetylcholinesterase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:19642642</ref><references group="xtra"/>
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__TOC__
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[[Category: Acetylcholinesterase]]
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</StructureSection>
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[[Category: Torpedo californica]]
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[[Category: Large Structures]]
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[[Category: Ashani, Y.]]
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[[Category: Tetronarce californica]]
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[[Category: Colletier, J P.]]
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[[Category: Ashani Y]]
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[[Category: Froment, M T.]]
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[[Category: Colletier JP]]
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[[Category: Greenblatt, H M.]]
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[[Category: Froment MT]]
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[[Category: Masson, P.]]
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[[Category: Greenblatt HM]]
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[[Category: Nachon, F.]]
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[[Category: Masson P]]
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[[Category: Sanson, B.]]
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[[Category: Nachon F]]
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[[Category: Silman, I.]]
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[[Category: Sanson B]]
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[[Category: Sussman, J L.]]
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[[Category: Silman I]]
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[[Category: Toker, L.]]
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[[Category: Sussman JL]]
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[[Category: Weik, M.]]
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[[Category: Toker L]]
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[[Category: Aging]]
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[[Category: Weik M]]
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[[Category: Alzheimer disease]]
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[[Category: Cell junction]]
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[[Category: Cell membrane]]
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[[Category: Glycoprotein]]
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[[Category: Gpi-anchor]]
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[[Category: Hydrolase]]
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[[Category: Neurotransmitter degradation]]
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[[Category: Synapse membrane]]
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Current revision

NON-AGED CONJUGATE OF TORPEDO CALIFORNICA ACETYLCHOLINESTERASE WITH SOMAN (ALTERNATIVE REFINEMENT)

PDB ID 2wg2

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