3oto

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[[Image:3oto.png|left|200px]]
 
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==Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8)==
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The line below this paragraph, containing "STRUCTURE_3oto", creates the "Structure Box" on the page.
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<StructureSection load='3oto' size='340' side='right'caption='[[3oto]], [[Resolution|resolution]] 3.69&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3oto]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3oto| PDB=3oto | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oto OCA], [https://pdbe.org/3oto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oto RCSB], [https://www.ebi.ac.uk/pdbsum/3oto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oto ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/3oto_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3oto ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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All organisms incorporate post-transcriptional modifications into ribosomal RNA, influencing ribosome assembly and function in ways that are poorly understood. The most highly conserved modification is the dimethylation of two adenosines near the 3' end of the small subunit rRNA. Lack of these methylations due to deficiency in the KsgA methyltransferase stimulates translational errors during both the initiation and elongation phases of protein synthesis and confers resistance to the antibiotic kasugamycin. Here, we present the X-ray crystal structure of the Thermus thermophilus 30S ribosomal subunit lacking these dimethylations. Our data indicate that the KsgA-directed methylations facilitate structural rearrangements in order to establish a functionally optimum subunit conformation during the final stages of ribosome assembly.
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===Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8)===
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Modification of 16S ribosomal RNA by the KsgA methyltransferase restructures the 30S subunit to optimize ribosome function.,Demirci H, Murphy F 4th, Belardinelli R, Kelley AC, Ramakrishnan V, Gregory ST, Dahlberg AE, Jogl G RNA. 2010 Oct 20. PMID:20962038<ref>PMID:20962038</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20962038}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3oto" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20962038 is the PubMed ID number.
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{{ABSTRACT_PUBMED_20962038}}
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==About this Structure==
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[[3oto]] is a 21 chain structure of [[Ribosomal protein S10]], [[Ribosomal protein S11]], [[Ribosomal protein S12]], [[Ribosomal protein S13]], [[Ribosomal protein S15]], [[Ribosomal protein S17]], [[Ribosomal protein S20]] and [[Ribosomal protein THX]] with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTO OCA].
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==See Also==
==See Also==
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*[[Ribosomal protein S10]]
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*[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]]
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*[[Ribosomal protein S11]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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*[[Ribosomal protein S12]]
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== References ==
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*[[Ribosomal protein S13]]
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<references/>
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*[[Ribosomal protein S15]]
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__TOC__
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*[[Ribosomal protein S17]]
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</StructureSection>
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*[[Ribosomal protein S20]]
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[[Category: Large Structures]]
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*[[Ribosomal protein THX]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Belardinelli R]]
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==Reference==
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[[Category: Dahlberg AE]]
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<ref group="xtra">PMID:20962038</ref><references group="xtra"/>
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[[Category: Demirci H]]
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[[Category: Thermus thermophilus]]
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[[Category: Gregory ST]]
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[[Category: Belardinelli, R.]]
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[[Category: Jogl G]]
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[[Category: Dahlberg, A E.]]
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[[Category: Kelley AC]]
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[[Category: Demirci, H.]]
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[[Category: Murphy IV F]]
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[[Category: Gregory, S T.]]
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[[Category: Ramakrishnan V]]
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[[Category: IV, F Murphy.]]
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[[Category: Jogl, G.]]
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[[Category: Kelley, A C.]]
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[[Category: Ramakrishnan, V.]]
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[[Category: 30s ribosomal subunit]]
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[[Category: Antibiotic resistance]]
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[[Category: Decoding]]
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[[Category: Decoding of genetic code]]
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[[Category: Ksga knock-out]]
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[[Category: Mrna]]
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[[Category: Post transcriptional rrna modification]]
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[[Category: Ribosome]]
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[[Category: Trna]]
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Current revision

Crystal Structure of the 30S ribosomal subunit from a KsgA mutant of Thermus thermophilus (HB8)

PDB ID 3oto

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