1uzv

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[[Image:1uzv.gif|left|200px]]<br />
 
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<applet load="1uzv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1uzv, resolution 1.00&Aring;" />
 
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'''HIGH AFFINITY FUCOSE BINDING OF PSEUDOMONAS AERUGINOSA LECTIN II: 1.0 A CRYSTAL STRUCTURE OF THE COMPLEX'''<br />
 
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==Overview==
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==High affinity fucose binding of Pseudomonas aeruginosa lectin II: 1.0 A crystal structure of the complex==
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PA-IIL is a fucose-binding lectin from Pseudomonas aeruginosa that is, closely related to the virulence factors of the bacterium. Previous, structural studies have revealed a new carbohydrate-binding mode with, direct involvement of two calcium ions (Mitchell E, Houles C, Sudakevitz, D, Wimmerova M, Gautier C, Perez S, Wu AM, Gilboa-Garber N, Imberty A., Structural basis for selective recognition of oligosaccharides from cystic, fibrosis patients by the lectin PA-IIL of Pseudomonas aeruginosa. Nat, Struct Biol 2002;9:918-921). A combination of thermodynamic, structural, and computational methods has been used to study the basis of the high, affinity for the monosaccharide ligand. A titration microcalorimetry study, indicated that the high affinity is enthalpy driven. The crystal ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15573375 (full description)]]
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<StructureSection load='1uzv' size='340' side='right'caption='[[1uzv]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1uzv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UZV FirstGlance]. <br>
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1UZV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with FUC, SO4 and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: C1A. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UZV OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uzv OCA], [https://pdbe.org/1uzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uzv RCSB], [https://www.ebi.ac.uk/pdbsum/1uzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uzv ProSAT]</span></td></tr>
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High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 A resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches., Mitchell EP, Sabin C, Snajdrova L, Pokorna M, Perret S, Gautier C, Hofr C, Gilboa-Garber N, Koca J, Wimmerova M, Imberty A, Proteins. 2005 Feb 15;58(3):735-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15573375 15573375]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9HYN5_PSEAE Q9HYN5_PSEAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uz/1uzv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uzv ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Budova M]]
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[[Category: Budova, M.]]
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[[Category: Gautier C]]
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[[Category: Gautier, C.]]
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[[Category: Gilboa-Garber N]]
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[[Category: Gilboa-Garber, N.]]
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[[Category: Imberty A]]
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[[Category: Imberty, A.]]
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[[Category: Koca J]]
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[[Category: Koca, J.]]
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[[Category: Mitchell E]]
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[[Category: Mitchell, E.]]
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[[Category: Perret S]]
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[[Category: Perret, S.]]
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[[Category: Sabin CD]]
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[[Category: Sabin, C.D.]]
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[[Category: Snajdrova L]]
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[[Category: Snajdrova, L.]]
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[[Category: Wimmerova M]]
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[[Category: Wimmerova, M.]]
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[[Category: CA]]
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[[Category: FUC]]
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[[Category: SO4]]
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[[Category: calcium]]
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[[Category: fucose]]
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[[Category: lectin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:21:44 2007''
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Current revision

High affinity fucose binding of Pseudomonas aeruginosa lectin II: 1.0 A crystal structure of the complex

PDB ID 1uzv

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