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2yt4

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[[Image:2yt4.gif|left|200px]]<br /><applet load="2yt4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2yt4, resolution 2.60&Aring;" />
 
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'''Crystal structure of human DGCR8 core'''<br />
 
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==Overview==
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==Crystal structure of human DGCR8 core==
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A complex of Drosha with DGCR8 (or its homolog Pasha) cleaves primary, microRNA (pri-miRNA) substrates into precursor miRNA and initiates the, microRNA maturation process. Drosha provides the catalytic site for this, cleavage, whereas DGCR8 or Pasha provides a frame for anchoring substrate, pri-miRNAs. To clarify the molecular basis underlying recognition of, pri-miRNA by DGCR8 and Pasha, we determined the crystal structure of the, human DGCR8 core (DGCR8S, residues 493-720). In the structure, the two, double-stranded RNA-binding domains (dsRBDs) are arranged with pseudo, two-fold symmetry and are tightly packed against the C-terminal helix. The, H2 helix in each dsRBD is important for recognition of pri-miRNA, substrates. This structure, together with fluorescent resonance energy, transfer and mutational analyses, suggests that the DGCR8 core recognizes, pri-miRNA in two possible orientations. We propose a model for DGCR8's, recognition of pri-miRNA.
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<StructureSection load='2yt4' size='340' side='right'caption='[[2yt4]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2yt4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YT4 FirstGlance]. <br>
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2YT4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YT4 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yt4 OCA], [https://pdbe.org/2yt4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yt4 RCSB], [https://www.ebi.ac.uk/pdbsum/2yt4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yt4 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of human DGCR8 core., Sohn SY, Bae WJ, Kim JJ, Yeom KH, Kim VN, Cho Y, Nat Struct Mol Biol. 2007 Sep;14(9):847-53. Epub 2007 Aug 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17704815 17704815]
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== Function ==
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[https://www.uniprot.org/uniprot/DGCR8_HUMAN DGCR8_HUMAN] Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self-renewal.<ref>PMID:15589161</ref> <ref>PMID:15574589</ref> <ref>PMID:15531877</ref> <ref>PMID:16751099</ref> <ref>PMID:16906129</ref> <ref>PMID:16963499</ref> <ref>PMID:17159994</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yt/2yt4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2yt4 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cho, Y.]]
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[[Category: Cho Y]]
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[[Category: Sohn, S.Y.]]
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[[Category: Sohn SY]]
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[[Category: dsrbd]]
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[[Category: rna binding domain]]
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[[Category: rna binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:41:38 2008''
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Current revision

Crystal structure of human DGCR8 core

PDB ID 2yt4

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