2qrc

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(New page: 200px<br /><applet load="2qrc" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qrc, resolution 2.70&Aring;" /> '''Crystal structure of...)
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[[Image:2qrc.jpg|left|200px]]<br /><applet load="2qrc" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qrc, resolution 2.70&Aring;" />
 
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'''Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with ADP and AMP'''<br />
 
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==Overview==
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==Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with ADP and AMP==
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The AMP-activated protein kinase (AMPK), a sensor of cellular energy, status found in all eukaryotes, responds to changes in intracellular, adenosine nucleotide levels resulting from metabolic stresses. Here we, describe crystal structures of a heterotrimeric regulatory core fragment, from Schizosaccharomyces pombe AMPK in complex with ADP, ADP/AMP, ADP/ATP, and 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (AICAR, phosphate, or ZMP), a well-characterized AMPK activator. Prior, crystallographic studies had revealed a single site in the gamma subunit, that binds either ATP or AMP within Bateman domain B. Here we show that, ZMP binds at this site, mimicking the binding of AMP. An analogous site in, Bateman domain A selectively accommodates ADP, which binds in a distinct, manner that also involves direct ligation to elements from the beta, subunit. These observations suggest a possible role for ADP in regulating, AMPK response to changes in cellular energy status.
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<StructureSection load='2qrc' size='340' side='right'caption='[[2qrc]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2qrc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QRC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qrc OCA], [https://pdbe.org/2qrc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qrc RCSB], [https://www.ebi.ac.uk/pdbsum/2qrc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qrc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SNF1_SCHPO SNF1_SCHPO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/2qrc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qrc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The AMP-activated protein kinase (AMPK), a sensor of cellular energy status found in all eukaryotes, responds to changes in intracellular adenosine nucleotide levels resulting from metabolic stresses. Here we describe crystal structures of a heterotrimeric regulatory core fragment from Schizosaccharomyces pombe AMPK in complex with ADP, ADP/AMP, ADP/ATP, and 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (AICAR phosphate, or ZMP), a well-characterized AMPK activator. Prior crystallographic studies had revealed a single site in the gamma subunit that binds either ATP or AMP within Bateman domain B. Here we show that ZMP binds at this site, mimicking the binding of AMP. An analogous site in Bateman domain A selectively accommodates ADP, which binds in a distinct manner that also involves direct ligation to elements from the beta subunit. These observations suggest a possible role for ADP in regulating AMPK response to changes in cellular energy status.
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==About this Structure==
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Structural insight into AMPK regulation: ADP comes into play.,Jin X, Townley R, Shapiro L Structure. 2007 Oct;15(10):1285-95. PMID:17937917<ref>PMID:17937917</ref>
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2QRC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with <scene name='pdbligand=AMP:'>AMP</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRC OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural Insight into AMPK Regulation: ADP Comes into Play., Jin X, Townley R, Shapiro L, Structure. 2007 Oct;15(10):1285-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17937917 17937917]
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</div>
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[[Category: Non-specific serine/threonine protein kinase]]
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<div class="pdbe-citations 2qrc" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Jin, X.]]
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[[Category: Jin X]]
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[[Category: Shapiro, L.]]
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[[Category: Shapiro L]]
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[[Category: Townley, R.]]
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[[Category: Townley R]]
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[[Category: ADP]]
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[[Category: AMP]]
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[[Category: adp]]
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[[Category: amp]]
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[[Category: ampk]]
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[[Category: atp-binding]]
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[[Category: cbs domain]]
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[[Category: kinase]]
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[[Category: nucleotide-binding]]
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[[Category: serine/threonine-protein kinase]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:44:34 2008''
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Current revision

Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with ADP and AMP

PDB ID 2qrc

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