2qre

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(New page: 200px<br /><applet load="2qre" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qre, resolution 3.01&Aring;" /> '''Crystal structure of...)
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[[Image:2qre.jpg|left|200px]]<br /><applet load="2qre" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qre, resolution 3.01&Aring;" />
 
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'''Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (ZMP)'''<br />
 
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==Overview==
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==Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (ZMP)==
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The AMP-activated protein kinase (AMPK), a sensor of cellular energy, status found in all eukaryotes, responds to changes in intracellular, adenosine nucleotide levels resulting from metabolic stresses. Here we, describe crystal structures of a heterotrimeric regulatory core fragment, from Schizosaccharomyces pombe AMPK in complex with ADP, ADP/AMP, ADP/ATP, and 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (AICAR, phosphate, or ZMP), a well-characterized AMPK activator. Prior, crystallographic studies had revealed a single site in the gamma subunit, that binds either ATP or AMP within Bateman domain B. Here we show that, ZMP binds at this site, mimicking the binding of AMP. An analogous site in, Bateman domain A selectively accommodates ADP, which binds in a distinct, manner that also involves direct ligation to elements from the beta, subunit. These observations suggest a possible role for ADP in regulating, AMPK response to changes in cellular energy status.
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<StructureSection load='2qre' size='340' side='right'caption='[[2qre]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2qre]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QRE FirstGlance]. <br>
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2QRE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with <scene name='pdbligand=AMZ:'>AMZ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRE OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMZ:AMINOIMIDAZOLE+4-CARBOXAMIDE+RIBONUCLEOTIDE'>AMZ</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qre FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qre OCA], [https://pdbe.org/2qre PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qre RCSB], [https://www.ebi.ac.uk/pdbsum/2qre PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qre ProSAT]</span></td></tr>
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Structural Insight into AMPK Regulation: ADP Comes into Play., Jin X, Townley R, Shapiro L, Structure. 2007 Oct;15(10):1285-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17937917 17937917]
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</table>
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[[Category: Non-specific serine/threonine protein kinase]]
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== Function ==
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[[Category: Protein complex]]
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[https://www.uniprot.org/uniprot/SNF1_SCHPO SNF1_SCHPO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/2qre_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qre ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Jin, X.]]
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[[Category: Jin X]]
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[[Category: Shapiro, L.]]
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[[Category: Shapiro L]]
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[[Category: Townley, R.]]
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[[Category: Townley R]]
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[[Category: AMZ]]
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[[Category: 5-aminoimidazole-4-carboxamide 1-beta-d-ribofuranotide]]
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[[Category: aicar phosphate]]
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[[Category: ampk]]
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[[Category: atp-binding]]
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[[Category: cbs domain]]
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[[Category: kinase]]
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[[Category: nucleotide-binding]]
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[[Category: serine/threonine-protein kinase]]
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[[Category: transferase]]
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[[Category: zmp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:44:40 2008''
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Current revision

Crystal structure of the adenylate sensor from AMP-activated protein kinase in complex with 5-aminoimidazole-4-carboxamide 1-beta-D-ribofuranotide (ZMP)

PDB ID 2qre

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