3ixt

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[[Image:3ixt.png|left|200px]]
 
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==Crystal Structure of Motavizumab Fab Bound to Peptide Epitope==
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The line below this paragraph, containing "STRUCTURE_3ixt", creates the "Structure Box" on the page.
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<StructureSection load='3ixt' size='340' side='right'caption='[[3ixt]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ixt]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus_A2 Human respiratory syncytial virus A2] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IXT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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{{STRUCTURE_3ixt| PDB=3ixt | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ixt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixt OCA], [https://pdbe.org/3ixt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ixt RCSB], [https://www.ebi.ac.uk/pdbsum/3ixt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ixt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FUS_HRSVA FUS_HRSVA] Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis.<ref>PMID:12663767</ref> <ref>PMID:18216092</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/3ixt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ixt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Motavizumab is approximately tenfold more potent than its predecessor, palivizumab (Synagis), the FDA-approved monoclonal antibody used to prevent respiratory syncytial virus (RSV) infection. The structure of motavizumab in complex with a 24-residue peptide corresponding to its epitope on the RSV fusion (F) glycoprotein reveals the structural basis for this greater potency. Modeling suggests that motavizumab recognizes a different quaternary configuration of the F glycoprotein than that observed in a homologous structure.
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===Crystal Structure of Motavizumab Fab Bound to Peptide Epitope===
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Structural basis of respiratory syncytial virus neutralization by motavizumab.,McLellan JS, Chen M, Kim A, Yang Y, Graham BS, Kwong PD Nat Struct Mol Biol. 2010 Feb;17(2):248-50. Epub 2010 Jan 24. PMID:20098425<ref>PMID:20098425</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20098425}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3ixt" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20098425 is the PubMed ID number.
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{{ABSTRACT_PUBMED_20098425}}
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==About this Structure==
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[[3ixt]] is a 6 chain structure of [[Monoclonal Antibody]] with sequence from [http://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus_a2 Human respiratory syncytial virus a2] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXT OCA].
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==See Also==
==See Also==
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*[[Monoclonal Antibody]]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:20098425</ref><references group="xtra"/>
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__TOC__
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[[Category: Human respiratory syncytial virus a2]]
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</StructureSection>
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[[Category: Human respiratory syncytial virus A2]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Chen, M.]]
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[[Category: Chen M]]
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[[Category: Graham, B S.]]
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[[Category: Graham BS]]
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[[Category: Kim, A.]]
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[[Category: Kim A]]
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[[Category: Kwong, P D.]]
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[[Category: Kwong PD]]
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[[Category: McLellan, J S.]]
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[[Category: McLellan JS]]
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[[Category: Yang, Y.]]
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[[Category: Yang Y]]
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[[Category: Cell membrane]]
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[[Category: Cleavage on pair of basic residue]]
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[[Category: Coiled coil]]
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[[Category: Complex]]
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[[Category: Disulfide bond]]
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[[Category: Envelope protein]]
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[[Category: Fab]]
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[[Category: Fusion protein]]
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[[Category: Glycoprotein]]
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[[Category: Immune system]]
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[[Category: Lipoprotein]]
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[[Category: Membrane]]
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[[Category: Monoclonal]]
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[[Category: Motavizumab]]
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[[Category: Palmitate]]
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[[Category: Rsv]]
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[[Category: Synagi]]
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[[Category: Transmembrane]]
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[[Category: Virion]]
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Current revision

Crystal Structure of Motavizumab Fab Bound to Peptide Epitope

PDB ID 3ixt

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